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Fishery Bulletin 111(3) 
Short time intervals (<3 days) In experiment 7, the 
planned time intervals between lifts were 1-2 days. 
However, because of ocean conditions, they ranged from 
1 to 4 days. The total number of pot lifts was 9, and 
hemolymph was not sampled after the third, fifth, and 
seventh lifts (Table 1). Large variations in [He] in the 
hemolymph of snow crab were observed after the sec- 
ond pot lift until the end of the experiment (Fig. 5). In 
one-third of experimental animals, [He] was markedly 
lower, by 50% or more, than mean [He] observed in con- 
trol crabs, although, in the remaining animals used in 
the experiment, [He] did not differ significantly from 
He levels in control samples. 
Experiments 5 and 6 were similar to but not as pro- 
longed as experiment 7. In both of these experiments, 
large variations in [He] in snow and southern Tanner 
crabs were observed. In experiment 5, in approximately 
Figure 5 
Changes in mean hemocyanin concentration [He] in 
snow crab (Chionoecetes opilio) during repeated pot 
hauls at short time intervals (<3 days) in experiments 
conducted on 17 October-3 November 2008 in area II 
of the Sea of Okhtosk to study the effects of commer- 
cial pot fishing on the physical condition of these spe- 
cies (see Fig. 2 for location of area II). In experiment 
7, ocean conditions extended time intervals to 1-4 days 
rather than the planned 1-2 days. Open bar indicate 
mean [He] observed in crabs sampled immediately af- 
ter capture (number of individuals was 12), and shaded 
bars indicate mean [He] observed in crabs sampled af- 
ter repeated pot hauls (the number of individuals sam- 
pled after each pot haul was 3-5). Error bars indicate 
±1 standard error of the mean. Arrows indicate third, 
fifth, and seventh pot lifts when sampling of blood was 
not carried out. 
one-half of individual snow crab, [He] was markedly 
lower, by 80% or more, than mean [He] in control crabs. 
In experiment 6, in approximately one-half of individual 
southern Tanner crab, [He] was markedly lower, by 70% 
or more, than mean [He] in control crabs. In both of 
these experiments, in one-half of experimental animals, 
[He] did not differ significantly from He levels in con- 
trol samples (data not shown). 
Effects of long-term starvation on crabs in pots 
In experiment 2 and 4, the duration of pot soaking was 
16 and 14 days, respectively. At the end of these ex- 
periments, mean [He] in hemolymph of snow crab and 
southern Tanner crab did not significantly differ from 
mean [He] in hemolymph of control crabs immediately 
after capture (data not shown). 
In experiment 8, the duration of pot soaking was 
25 days. At the end of this experiment, mean [He] in 
hemolymph of southern Tanner crab was slightly lower 
than [He] in control crab immediately after capture, but 
the mean difference between experimental and control ani- 
mals was not significant (Student’s t-test, P>0.05) (Fig. 6A). 
