Long and Van Sant: Embryo development in golden king crab ( Lithodes aequispinus) 
75 
t f— -I I 
-10 -5 0 5 
PCI 
Figure 6 
Plot of principal component analysis (PCA) of morphometries of golden king crab ( Lithodes 
aequispinus) embryos during 13 stages of development. Each point represents a single embryo. 
The first 2 PCs (plotted here ) accounted for 93% of the variance in the data. Vectors are shown 
for egg, embryo, yolk, and eye areas. 
Stage 
▲ 0 
▼ 1 
2 
♦ 3 
• 4 
+ 5 
X 6 
* 7 
A 8 
V 9 
□ 10 
O 11 
O 12 
crab were collected for this experiment. We thank B. 
Stevens for training, sharing techniques, and helpful 
suggestions, K. Reppond for assistance; and R. Foy for 
discussions. We also thank the staff, interns, and volun- 
teers of the seawater laboratory complex of the Kodiak 
Laboratory, NOAA Alaska Fisheries Science Center, for 
assistance in performing experiments. Previous ver- 
sions of this article were improved by comments from 
R. Foy, J. Long, K. Swiney, and 3 anonymous reviewers. 
The metadata associated with this project are avail- 
able at InPort (website) under the catalog item ID 
26894 and the title “AFSC/RACE/SAP/Long: Data from: 
Embryo development in golden king crab, Lithodes 
aequispinus.” The data are available at the following 
website. 
Literature cited 
Blau, S. F., D. Pengilly, and D. A. Tracy. 
1996. Distribution of golden king crabs by sex, size, and 
depth zones in the Eastern Aleutian Islands, Alaska. 
In High latitude crabs: biology, management, and eco- 
nomics. Alaska Sea Grant College Program Rep. AK- 
SG-96-02, p. 167-185. Univ. Alaska Fairbanks, Fair- 
banks, AK. 
Butler, T., and J. F. Hart. 
1962. The occurrence of the king crab, Paralithodes 
camtschatica (Tilesius), and of Lithodes aequispina 
Benedict in British Columbia. J. Fish. Res. Board Can. 
19:401-408. 
Clarke, K. R., and R. M. Warwick. 
2001. Change in marine communities: an approach to 
statistical analysis and interpretation, 2 nd ed, 172 p. 
PRIMER-E, Plymouth, U.K. 
Donaldson, W. E., and S. C. Byersdorfer. 
2005. Biological field techniques for lithodid crabs, 76 p. 
Univ. Alaska Fairbanks, Alaska Sea Grant College Pro- 
gram Rep. AK-SG-05-03. 
Haynes, E. 
1968. Relation of fecundity and egg length to carapace 
length in the king crab, Paralithodes camtschatica. Proc. 
Natl. Shellfisheries Assoc. 58:60-62. 
Hiramoto, K. 
1985. Overview of the golden king crab, Lithodes aequispi- 
na, fishery and its fisheries biology in the Pacific waters 
of Central Japan. In Proceedings of the International 
King Crab Symposium, Alaska Sea Grant College Pro- 
gram AK-SG-85-12; Anchorage, AK, 22-24 January, p. 
297-317. Univ. Alaska Fairbanks, Fairbanks, AK. 
Jewett, S. C., N. A. Sloan, and D. A. Somerton. 
1985. Size at sexual maturity and fecundity of the fjord- 
dwelling golden king crab Lithodes aequispina Bene- 
dict from northern British Columbia. J. Crust. Biol. 
5:377-385. 
Long, W. C. 
2016. A new quantitative model of multiple transitions 
between discrete stages, applied to the development of 
crustacean larvae. Fish. Bull. 114:58-66. 
