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Fishery Bulletin 1 13(4) 
Table 2 
Analysis of molecular variance (AMOVA) of dolphinfish (Coryphaena hippurus ) based on mitochondrial nico- 
tinamide adenine dinucleotide (NADH) dehydrogenase subunit 1 (ND1) sequences by region in the western 
central Atlantic. Total population differentiation was estimated by using maximum likelihood DNA sequence 
pairwise distances (Tamura-Nei correction). The fixation indices for differentiation within a population among 
samples (<t>sT=0 009> P=0.023), among regions (0cy), and among areas within regions (<figc) are presented. 
The areas and regions in this study were the northern and southern coasts of Puerto Rico in the northeastern 
Caribbean Sea; Florida, South Carolina, and North Carolina within the southeastern United States; Domi- 
nica, Barbados, and Trinidad and Tobago in the eastern Caribbean Sea; and the Azores Islands in the central 
North Atlantic. 
Source of variation 
Sum of squares Variance Variation (%) Fixation indices 
Among regions 8.179 
Among areas within regions 17.997 
Within areas 775.105 
Total 801.281 
-0.1354 -0.55 <Z> C T= —0.005 (P=0. 301) 
0.3509 1.41 <fi S C=0.014 (P=0.059) 
2.4607 99.13 
2.4822 
A B 
Number of pairwise differences 
Figure 3 
Number of pairwise differences from mismatch distributions of dolphinfish (Coryphaena 
hippurus ) based on analyses of mitochondrial nicotinamide adenine dinucleotide (NADH) 
dehydrogenase subunit 1 ( ND1) sequence data from fish sampled in the (A) southeast- 
ern United States in 2012, (B) northeastern Caribbean Sea in 2010-2013, (C) eastern 
Caribbean Sea in 1998 and 2014, and (D) central North Atlantic in 1998. Bars indicate 
the observed distributions under the spatial expansion model. The curve indicates the 
simulated distribution. 
