444 
Fishery Bulletin 113(4) 
Figure 1 
Capture locations for the specimens of hogfish ( Lachnolaimus maximus) that were used to genetically determine the popula- 
tion structure within the southeastern United States. Specimens were collected sporadically between November 2005 and 
August 2013 and were grouped by collection location into sampling areas 1-9, where l=Big Bend, 2=Nature Coast and Florida 
Middle Grounds, 3=Tampa Bay, 4=Sarasota, 5=Naples, 6=Everglades, 7=Florida Keys, 8=East Florida, 9=Carolinas. Three 
distinct clusters were identified as the 1) eastern Gulf of Mexico; 2) Florida Keys and southeast Florida; and 3) Carolinas, on 
the basis of the genetically determined population structure detected with 24 microsatellite loci. Sampling area 6 was identi- 
fied as the region of gene flow restriction (genetic break) between clusters 1 and 2. 
the Suwannee River (between sampling areas 1 and 2), 
Tampa Bay (in sampling area 3), and Charlotte Harbor 
(in sampling area 4). In terms of management jurisdic- 
tions, the Gulf of Mexico Fishery Management Council 
regulates federal waters throughout the Gulf of Mexico 
(which includes sampling areas 1-6), and the South At- 
lantic Fishery Management Council regulates federal 
waters from the Florida Keys through the Carolinas 
(an area that includes sampling areas 7-9). The state 
of Florida regulates state waters within 14.5 km (9 mi) 
from shore in the Gulf of Mexico and 4.9 km (3 mi) 
from shore in the Atlantic Ocean. 
Microsatellite genotyping 
Specimens were genotyped by using 24 of the 29 micro- 
satellite markers identified in MERPDC (2012); mark- 
ers Lmaxll, Lmaxl4, Lmaxl5, Lmax24, and Lmax31 
were not used. Multiplex polymerase chain reaction 
amplifications were carried out in a Mastercycler Pro 
thermal cycler (Eppendorf North America, Hauppauge, 
NY) containing 50-100 ng of total DNA, 10 pL of 50 
pM dNTP mix, 0.25 pL of 0.1 mg/mL bovine serum al- 
bumin, a combination of 3 optimally selected primers 
of 3 loci with each forward primer labeled with a dif- 
ferent fluorescent dye, 5 pL of Taq polymerase lOx buf- 
fer (Promega, Madison, WI) containing 15 mM MgCl 2 
and 1.25 units of Taq polymerase (Promega). The reac- 
tion profile was 94°C for 2 min, 35 cycles of 94°C for 
35 s, 55°C for 35 s, 72°C for 35 s, and final extension 
at 72°C for 30 min. Fragments were visualized on an 
Applied Biosystems 3130 XL genetic analyzer (Thermo 
Scientific Inc., Waltham, MA) and genotyped with Gen- 
eMapper software, vers. 4.0 (Thermo Scientific Inc.). 
For fragment assays, we used GeneScan 500 ROX Size 
Standard (Thermo Scientific Inc.). 
