Darden et al.: Population genetics of Rachycentron canadum 
31 
Table 5 ( continued ) 
2005 
M2211 
05/03/06 
05/19/06 
Port Royal Sound 
16 
11.0 
2005 
M2028 
04/27/06 
05/14/06 
St. Helena Sound 
17 
62.0 
2005 
M2102 
05/03/06 
05/20/06 
- 
17 
- 
2005 
M2086 
05/03/06 
05/21/06 
Port Royal Sound 
18 
11.0 
2005 
M2107 
05/03/06 
05/21/06 
Port Royal Sound 
18 
11.0 
2005 
M2146 
05/03/06 
05/21/06 
Port Royal Sound 
18 
11.0 
2005 
M2007 
04/27/06 
05/16/06 
Port Royal Sound 
19 
11.0 
2005 
M2187 
05/03/06 
05/22/06 
Port Royal Sound 
19 
11.0 
2005 
M2005 
04/27/06 
05/17/06 
Port Royal Sound 
20 
11.0 
2005 
M2042 
04/27/06 
05/17/06 
Port Royal Sound 
20 
11.0 
2005 
M2002 
04/27/06 
05/19/06 
Port Royal Sound 
22 
11.0 
2005 
M2232 
04/27/06 
05/19/06 
Port Royal Sound 
22 
11.0 
2005 
M2016 
04/27/06 
05/21/06 
Port Royal Sound 
24 
- 
2005 
M2039 
04/27/06 
05/21/06 
Port Royal Sound 
24 
- 
2005 
M2163 
05/03/06 
05/29/06 
- 
26 
- 
2005 
M2223 
05/03/06 
05/29/06 
- 
26 
- 
2005 
M2241 
04/27/06 
05/24/06 
Port Royal Sound 
27 
- 
2005 
M2068 
05/03/06 
06/05/06 
- 
33 
- 
2005 
M2141 
05/03/06 
06/05/06 
St. Helena Sound 
33 
54.7 
2005 
M2015 
04/27/06 
06/02/06 
Port Royal Sound 
36 
11.0 
2005 
M2027 
04/27/06 
06/02/06 
Port Royal Sound 
36 
11.0 
2005 
M2242 
04/27/06 
06/06/06 
Port Royal Sound 
40 
11.0 
2005 
M2377 
05/03/06 
05/05/07 
Charleston Harbor 
367 
123.9 
2005 
M2249 
05/03/06 
09/15/07 
Offshore SC 
500 
65.5 
2005 
M2131 
05/03/06 
05/20/08 
Port Royal Sound 
748 
11.0 
2005 
M2238 
04/27/06 
05/20/08 
Calibogue Sound 
754 
44.3 
2005 
M2017 
04/27/06 
05/25/08 
Port Royal Sound 
759 
20.6 
2005 
M2281 
04/27/06 
05/30/08 
Port Royal Sound 
764 
11.0 
2005 
M217? 
05/03/06 
06/18/08 
Port Royal Sound 
777 
20.0 
2005 
M2046 
04/27/06 
06/18/08 
Port Royal Sound 
783 
20.0 
2005 
M2197 
05/03/06 
10/03/08 
Offshore SC 
884 
77.2 
2005 
M2118 
05/03/06 
05/11/09 
Offshore FL 
1104 
241.4 
2005 
M2387 
05/03/06 
05/15/09 
Port Royal Sound 
1108 
11.0 
2005 
M2265 
05/03/06 
09/14/09 
St. Mary’s River, FL 
1230 
207.6 
2005 
M2004 
04/27/06 
- 
- 
- 
- 
2005 
M2051 
05/03/06 
- 
- 
- 
- 
Table 6 
Summary of results for the comparision of genotypic 
distribution (P-values below diagonal) and f?sT pairwise 
(above diagonal) location from the 2008 sample collec- 
tions of Cobia ( Rachycentron canadum). Collection lo- 
cations included inshore Virginia (VA), offshore North 
Carolina (NCoff), and offshore South Carolina (SCoff), 
and inshore South Carolina (SC). Bold type indicates 
statistical significance after Bonferroni correction (criti- 
cal P=0.008). 
VA 
NCoff 
SCoff 
sc 
VA 
0.001 
0.011 
0.043 
NC 0 ff 
0.013 
- 
0.005 
0.023 
SC 0 fF 
<0.001 
0.075 
- 
0.016 
SC 
<0.001 
<0.001 
<0.001 
- 
collection and 2 populations for the 2009 collections. 
Consistent with the low estimates, the results 
from the Structure software indicated the presence of 
mixed ancestry among these populations. 
Temporal within-location comparisons of the 2008 
and 2009 collections showed no significant differ- 
ences in genetic compositions (G-test: P=0.51-0.56; 
f?ST<0 006, P>0.016), validating the pooling of samples 
across collection years for population characterization. 
Basic molecular diversity indices were similar among 
collection locations, with high levels of genetic diver- 
sity across all loci and high levels of polymorphism 
(Table 9). The mean number of alleles per locus ranged 
from 10.4 to 14.0 (average allelic range: 14.5-16.4). The 
overall average observed heterozygosity for Cobias was 
moderate and ranged from 0.709 in the SC inshore col- 
lection to 0.772 in the VA inshore collection, with the 
NC and SC offshore collections showing intermediate 
