246 
Fishery Bulletin 1 10(2) 
Table 3 
Number of alleles per locus, an index of genetic differentiation F ST (standard deviation in parentheses), expected heterozygosity 
(H e ), observed heterozygosity (H 0 ), and percent significant Hardy-Weinberg equilibrium (HWE) tests for 16 microsatellite loci 
among 146 odd-year and 116 even-year pink salmon (Oncorhynchus gorbuscha) populations. 
Locus 
Number of alleles 
1 
ST 
H e 
H 0 
HWE 
Odd-year broodline 
OkilO 
85 
0.004 
(0.000) 
0.94 
0.94 
0.0 
OkilOl 
83 
0.002 
(0.000) 
0.97 
0.96 
0.7 
OnelOl 
52 
0.003 
(0.000) 
0.96 
0.95 
0.7 
One 102 
32 
0.006 
(0.001) 
0.92 
0.91 
0.0 
One 104 
40 
0.007 
(0.001) 
0.95 
0.94 
0.0 
Onel09 
32 
0.005 
(0.001) 
0.90 
0.90 
0.0 
Onelll 
36 
0.012 
(0.001) 
0.72 
0.71 
0.0 
Onell4 
49 
0.003 
(0.000) 
0.96 
0.95 
2.1 
Ots213 
69 
0.003 
(0.001) 
0.97 
0.95 
2.8 
Ots7e 
12 
0.025 
(0.003) 
0.45 
0.45 
0.0 
OtsG253b 
46 
0.005 
(0.001) 
0.95 
0.94 
1.4 
OtsGSll 
34 
0.009 
(0.001) 
0.89 
0.88 
1.4 
OtsG68 
34 
0.004 
(0.001) 
0.93 
0.93 
0.0 
Ssa407 
73 
0.006 
(0.001) 
0.94 
0.94 
0.0 
Ssa408 
69 
0.002 
(0.000) 
0.97 
0.95 
6.2 
Ssa419 
66 
0.003 
(0.000) 
0.96 
0.93 
7.5 
Total 
0.005 
(0.001) 
Even-year broodline 
OkilO 
83 
0.001 
(0.000) 
0.94 
0.93 
1.8 
OkilOl 
73 
0.002 
(0.000) 
0.97 
0.96 
0.9 
OnelOl 
65 
0.001 
(0.000) 
0.96 
0.95 
1.8 
One 102 
35 
0.002 
(0.000) 
0.93 
0.93 
0.0 
Onel04 
36 
0.004 
(0.000) 
0.92 
0.92 
0.0 
Onel09 
29 
0.003 
(0.000) 
0.91 
0.90 
0.0 
Onelll 
38 
0.004 
(0.001) 
0.92 
0.91 
2.6 
Onell4 
42 
0.002 
(0.000) 
0.96 
0.95 
0.0 
Ots213 
71 
0.002 
(0.000) 
0.97 
0.97 
5.1 
Ots7e 
13 
0.005 
(0.001) 
0.58 
0.58 
1.8 
OtsG253b 
48 
0.002 
(0.000) 
0.95 
0.94 
3.5 
OtsG311 
34 
0.003 
(0.000) 
0.93 
0.91 
0.0 
OtsG68 
32 
0.003 
(0.000) 
0.93 
0.93 
0.0 
Ssa407 
77 
0.002 
(0.000) 
0.96 
0.93 
2.6 
Ssa408 
69 
0.002 
(0.000) 
0.97 
0.95 
1.8 
Ssa419 
66 
0.002 
(0.000) 
0.97 
0.95 
2.6 
Total 
0.002 
(0.000) 
Individual locus F ST values ranged from 0.002 to 0.012 
for the odd-year broodiine, and between 0.001 and 
0.005 for the even-year broodline. For the odd-year 
broodline, individual pairs of loci displayed poten- 
tial linkage of between 0% and 28% ( Ssa408 and 
Ssa419) of the populations surveyed. In the even-year 
broodline, individual pairs of loci displayed potential 
linkage of between 0% and 13% ( Ssa407 and Ssa419 ) 
of the populations surveyed. Potential linkage among 
loci was higher in the Ssa series of loci than that 
observed in the other microsatellites, but different 
patterns were observed within the broodlines. Poten- 
tial linkage among the Ssa loci was not judged to 
be at a level that required removal from subsequent 
analyses. 
The number of alleles observed displayed variation 
among the regional groups of pink salmon surveyed. 
With the number of alleles observed standardized to 
a sample size of 240 individuals per region for both 
broodlines, the odd-year broodline populations with 
the fewest number of observed alleles originated from 
Washington (486 alleles), whereas populations with the 
greatest number of alleles originated from the north- 
ern coastal region of British Columbia (551 alleles) 
(Table 4). In the even-year broodline, populations with 
the fewest numbers of alleles originated from the east 
