545 
ON INOCULATION WITH THE MATTER OF TYPHUS, 
DURING THE PREVALENCE OF EPIDEMIC 
TYPHOID DISEASES. 
By Professor DELAFOND, Royal Veterinary School, Alfort. 
[Continued from vol. x, page 373.] 
[We beg to apologise to our readers for the long delay in the 
continuation of one of the most valuable papers, in any language, 
on the Typhoid Epidemic Diseases of Quadrupeds. — Y.] 
A. The choice of the Virus, or Matter for Inoculation.- — The 
nasal discharge, the spume that gathers about the lips, the muco- 
puriform matter from the eye, the tears, and the sero-purulent fluid 
from the cutaneous pustules, have all been tried. 
The Grand Senechal, Claus Detlof, and Bulow, insist that the 
matter shall be obtained from animals affected with typhus of a mild 
character. 
Camper, Munnicks, and Vicq. d’Asyr, do not esteem this a matter 
of any consequence : but think that the intensity of the disease is 
regulated by the state of the inoculated animal, or by other accessory 
circumstances, and never by that of the beast from which the 
virus was taken. The disease, however, should not be at the state 
of greatest intensity. Recent matter is preferable to that which 
has been kept twenty-four hours, and, if it is used before it becomes 
cold, its effect is more certain. 
B. The Preservation of the Virus. — Munnicks has found that 
a thread, saturated with the virus, and enclosed in a sealed bottle, 
begins, about the fourth day, to acquire a musty smell, and is not 
proper for inoculation. Enclosed in a bottle hermetically sealed, and 
kept in a cold place, it retains its virtue during eight days. The 
air having been pumped out by means of a pneumatic machine, the 
empoisoned thread will do its duty after eleven or twelve days have 
elapsed, whether the virus has been taken from the nostrils or any 
other part. 
Detlof has preserved the virus from four to five days in the sum- 
mer, and fourteen days in the winter. The animal matter from the 
trenches in which the dead bodies were thrown preserved its 
contagious property the longest. Vicq. d’Asyr has communicated 
the disease by means of threads saturated with the putrid sanies 
that has lain in the trench more than three months. By means of 
tubes or small plates of glass, the matter is preserved during a 
period, in most cases, sufficiently long. 
