Lowe et al.: Geographic variation in genetic and growth patterns of Pleurogrammus monopterygius 
505 
Table 2 
Enzymes examined for allozyme variation. Enzyme commission (EC) numbers follow the IUBMBNC (1992). Tissues examined 
were muscle (M) and liver (L). Buffers used are described by Aebersold et al. (1987) except for the ACEN7 gel, which is an ACE7 
gel with 0.015% beta-nicotinamide adenine dinucleotide added to the gel and which has cathodal electrode buffers. 
Enzyme name 
EC Number 
Locus 
Tissue 
Buffer 
BetauV-Acetylgalactosaminidase 
3.2.1.53 
bGALA* 
L 
ACE7 
IV-Acetyl-beta-glucosaminidase 
3.2.1.30 
bGLUA* 
L 
ACE7 
Aconitate hydratase 
4.2. 1.3 
AH-1* 
M 
ACE7 
AH -2* 
L 
ACE 7 
Adenosine deaminase 
3. 5.4.4 
ADA* 
M 
TBE 
Adenylate kinase 
2.7. 4.3 
AK* 
M 
ACE7 
Alanine aminotransferase 
2. 6.1. 2 
ALAT* 
M 
TBE 
Alcohol dehydrogenase 
1.1. 1.1 
ADH* 
L 
ACE7 
Aspartate aminotransferase 
2.6.1. 1 
AAT-1* 
M 
ACE7 
AAT-2* 
L 
TBCLE, TBE 
AAT-3 * 
M 
TBCLE, TBE 
Creatine kinase 
2. 7. 3. 2 
CK* 
M 
TBCLE 
Esterase 
3.1.1.- 
EST* 
M 
TBCLE 
Enolase 
4.2.1.11 
ENO * 
M 
ACE7 
Fumarate hydratase 
4.2.1. 2 
FH* 
M 
ACEN7 
Glucose-6-phosphate isomerase 
5. 3.1.9 
GPI-1* 
M 
TBCLE 
GPI-2* 
M 
TBCLE 
Glycerol-3-phosphate dehydrogenase 
1.1. 1.8 
G3PDH-1* 
M 
ACEN7 
(NAD + ) 
G3PDH-2* 
M 
ACEN7 
L-Iditol dehydrogenase 
1.1.1.14 
IDDH* 
L 
TBCL 
Isocitrate dehydrogenase 
1.1.1.42 
IDHP-1* 
L 
ACE 7 
CNADP + ) 
IDHP-2* 
M 
ACE7 
L-Lactate dehydrogenase 
1.1.1.27 
LDH* 
M 
ACE7 
Malate dehydrogenase 
1.1.1.37 
mMDH* 
M 
ACEN7 
sMDH-1* 
ML 
ACEN7 
sMDH-2* 
M 
ACEN7 
Mannose-6-phosphate isomerase 
5.3. 1.8 
MPI* 
M 
TBE 
Peptidase-C 
3.4.-.- 
PEP-C * 
M 
TBE 
Phosphoglucomutase 
5. 4. 2. 2 
PGM* 
M 
TBCLE, ACE7 
Phosphogluconate dehydrogenase (decarboxylating) 
1.1.1.44 
PGDH* 
M 
ACE7 
Phosphoglycerate kinase 
2. 7. 2.3 
PGK* 
M 
ACE7 
Proline dipeptidase 
3.4.13.9 
PEPD* 
M 
TBE 
Pyruvate kinase 
2.7.1.40 
PK* 
M 
ACE7 
Superoxide dismutase 
1.15.1.1 
SOD* 
L 
ACE7 
Triose-phosphate isomerase 
5. 3.1.1 
TPI* 
M 
TBE 
Tripeptide aminopeptidase 
3.4.11.4 
PEPB-1* 
M 
TBE, TC4 
PEPB-2* 
M 
TBE 
the length-weight AN OVA included length and area 
factors and a length x area interaction term. 
Genetic data 
Genetic samples were collected from four locations 
throughout the Aleutian Archipelago (Fig. 1). Sample 
sizes were as follows: Attu Island, n=74; Kiska Is- 
land, n=80; Seguam Pass, n=lG0; and Umnak Island, 
n= 75. Muscle and liver tissues were collected from 
each specimen for protein electrophoresis and placed 
in the onboard freezers until they could be trans- 
ferred to a freezer set at -80°C. 
Genetic variability was examined by using starch 
gel electrophoresis as described by Aebersold et al. 
(1987). Initially, we examined 36 enzyme systems, 
of which 28 were clearly resolved. Those 28 enzyme 
systems revealed 37 loci. Tissue and buffer combi- 
nations for each enzyme are listed in Table 2. No- 
menclature for loci and alleles followed Shaklee et 
al. (1990). 
Genetic variability within samples was estimated 
as average heterozygosity (mean proportion of het- 
erozygous loci per individual), the percentage of poly- 
morphic loci per sample at the P () 95 level (frequency 
of the common allele <0.95), and the average num- 
