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Fishery Bulletin 96(3), 1998 
Table 2 
Allozyme protocols to resolve enzyme coding loci in Chionoecetes samples. Enzyme nomenclature follows Shaklee et al. (1990), 
and locus abbreviations are given. Tissue abbreviations are: (M) muscle, (H) heart, (G) gill, and (P) hepatopancreas. Buffers are 
described in the text. 
Enzyme or protein 
Enzyme 
number 
Locus 
Tissue 
Buffer 
Aspartate aminotransferase 
2.6.1. 1 
AAT-1*, AAT-2* 
M 
TBE 
Aconitate hydratase 
4.2. 1.3 
AH-2* 2 
M 
TC7.0 
AH-3* 
M 
TC7.0 
Adenosine deaminase 
3. 5. 4.4 
ADA-1*, ADAS* 
G,M,H 
TBCL 
ADAS* 
P 
TBCL 
Alanine aminotransferase 
2.6. 1.2 
ALAT* 
M 
TBCL 
Cytochrome-b5 2 
1.8. 1.4 
CBYR* 
M 
TBCL 
p- N - Acetylgalactosaminidase 
3.2.1.53 
PGALA* 
G,P 
TBCL, TBE 
Glyceraldehyde-3-phosphate dehydrogenase 
1.2.1.12 
GAPDH* 
M 
AC6.1 
N-Aeetyl-/)-glucosaminidase 
3.2.1.30 
PGLUA* 
G,P 
TBCL 
Glycerol-3-phosphate dehydrogenase 
1.1. 1.8 
G3PDH-1* 
M 
TC7.0 
G3PDH-2* 
H,M 
TC7.0 
Glucose-6-phosphate isomerase 
5.3. 1.9 
GPI-A1* 
M 
TBCL 
Isocitrate dehydrogenase (NADP+) 
1.1.1.42 
IDHP-1* 
H 
TC7.0 
Malate dehydrogenase 
1.1.1.37 
MDH-A1*, MDH-A2* 
M 
AC6.1 
Malic enzyme (NADP+) 
1.1.1.40 
MEP-1* 
H 
TC7.0 
Mannose-6-phosphate isomerase 
5.3. 1.8 
MPI* 
M 
TBE 
Dipeptidase 
3.4.-.- 
PEPA* 
H,P 
TBE 
Proline dipeptiase 
3.4.13.9 
PEPD-2* 
G,P 
TBCL 
Phosphogluconate dehydrogenase 
1.1.1.44 
PGDH* 
H 
TC7.0 
Phosphoglucomutase 
5.4.2. 2 
PGM-1* 
M,H 
TC7.0 
General (unidentified) protein 
PROT-1*, PROT-2*, PROT-3* 
M 
TBCL 
Superoxide dismutase 
1.15.1.1 
SOD-1* 2 
M 
TBCL 
Triose-phosphate isomerase 
5.3. 1.1 
TPI-1* 
M 
TBE 
1 This locus expressed with both CBYR* and GR*; preferential stain is CBYR* 
2 Used in C. opilio statistical analysis only. 
test significance of F gT values (2000 permutations). 
Allele frequencies were used to generate distance 
matrices of Cavalli-Sforza and Edwards chord dis- 
tances (Cavalli-Sforza and Edwards, 1967). Compu- 
tations were made with S-Plus analytical software 
(MathSoft Inc., 1997). 
Results 
Chionoecetes bairdi 
Twenty-seven loci were scored consistently in C. bairdi 
populations and were used in the data analyses. Tem- 
poral variation was examined for multiple-year collec- 
tions of C. bairdi from Bristol Bay, the Bering Sea and 
Pribilof Islands area, and Seymour Canal (Table 1). No 
significant interannual differences (P<0.01 ) were found 
within each geographic location; therefore these col- 
lections were pooled for subsequent analyses. 
Fifteen loci, AAT-1*, AAT-2*, AH-3*, ALAT *, 
CBYR*, G3PDH-1*, G3PDH-2*, GPI-A 1 *, IDHP-1*, 
MDH-A1*, PEPA*, PGDH*, PGM-1 *, PROT-3* , and 
TPI-1* were polymorphic (Table 3). At four loci, 
G3PDH-1*, GPI-A1 *, IDHP-1 *, and PEPA*, the most 
common allele had a frequency of <0.95 in at least 
one population. Twelve monomorphic loci, ADA-1*, 
ADA-2*, ADA-3*, pGALA*, GAPDH*, pGLUA*, 
MDH-A2*, MEP-1 *, MPI*, PEPD-2*, PROT-1 * , and 
PROT-2* were included in the 27-locus analyses. 
Seven monomorphic loci, AH-2*, mMDH-1* , PEPD- 
1*, SOD-1*, SOD-2*, TPI-2*, and XO* were not 
scorable in all populations and were not included in 
our analyses. We could not interpret the genetic ba- 
sis for the variation we observed in two consistently 
resolved zones of esterase activity; therefore those 
data were excluded. 
Three collections of C. bairdi. Sand Point and Pavlof, 
Port Moller, and Prince William Sound, with sample 
sizes of <25 at informative loci, AH-3* and IDHP-1*, 
were not included in the population data analyses; how- 
ever, we report allele frequencies in Table 3. 
Genotype frequencies at all loci conformed to Hardy- 
Weinberg expectations; therefore we assumed all sam- 
