660 
Fishery Bulletin 96(4), 1998 
Ba (F=46.85, P0.0001) 
Ca 
Fe 
* 
o> 
E 
More-1Herv-1Herv-3Bow-3 Inn-3 
Area 
K (F=4.58, P<0.002) 
43 
40 
37 
12 
More-1Herv-1Herv-3Bow-3 Inn-3 
Area 
Li 
More-1 Herv-1 Herv-3 Bow-3 Inn-3 
Area 
Mg (F=34.89, P<0 0001) 
800 
600 
c 400 
S 
o 
O 
Mn 
8 
c 
o 
O 
More-1Herv-1 Herv-3 Bow-3 Inn-3 
Area 
28 
22 
§ 
<o 
f 16 
More-1Herv-1Herv-3Bow-3 Inn-3 
Area 
Na (F=52.80, P<0.0001) 
More-1Herv-1Herv-3Bow-3 Inn-3 
Area 
P (F=61 28, P<0 0001) 
More-1Herv-1Herv-3Bow-3 Inn-3 
Area 
More-1Herv-1Herv-3Bow-3 Inn-3 
Area 
_ 560 
CJ) 
S 500 
e 
■M 
S 
t 440 
S (F=18 58, P<0.0001) 
More-1Herv-1 Herv-3 Bow-3 Inn-3 
Area 
Sr (F=55.76, P<0.0001) 
Area 
Area 
Figure 6 
Mean elemental concentrations (± standard deviation) of spotted mackerel otoliths sampled from the differ- 
ent areas and F-values determined from AN OVA showing significant elements among samples (df=4, 140). 
Minimum elemental detection limits of the ICP-AES (all units are in mg/Kg, except Ca which is measured as a %): 
Ba = 0.78; Ca = 544; Fe = 23.9; K = 353; Li = 15.0; Mg = 10.9; Mn = 1.10; Na = 1250; P = 54.1; S = 191; Sr = 3.24. 
tween area of collection and fish length (ANCOVA, 
F- 3.87, df=4, 137, P<0.005). Manganese concentra- 
tion increased linearly with fish length for Hervey 
Bay 1-year-old fish, in contrast with the other 
samples that all showed negative correlations. 
Spotted mackerel samples from each area and for 
each age were found to have significant differences 
in mean elemental composition of the otoliths 
(MANOVA, Pillai’s trace=1.316, F=9.59, df=28, 548, 
PcO.0001). Little variation was evident in mean ele- 
