Small et al.: Population and stock indentification of Oncorhynchus kisutch 
845 
Figure 1 
Map of British Columbia showing locations of coho salmon samples. Population numbers are placed near the waterway 
(region or river system name in bold type precedes population names) : lower Fraser River 1) Chilliwack River; 2) 
Chehalis River; 3) Stave River; 4) Upper Pitt River; 5) Nicomen Slough; 6) Norrish Creek; 7) Inch Creek; upper Fraser 
River 8) Bridge River; Thompson River 9) Coldwater River; 10) Salmon River; 11) Eagle River; 12) Spius Creek; 13) 
Lemieux Creek; 14) Dunn Creek; 15) Louis River; 16) Deadman River; East Coast Vancouver Island 17 ) Big Qualicum 
River; 18) Quinsam River; North Coast Vancouver Island 19) Cluxewe River; 20) Stephens Creek; 21) Waukwaas 
Creek; West Coast Vancouver Island 22) Robertson Creek; 23) Nitinat River; Central Coast 24) Atnarko River; 25) 
Kitimat River; Queen Charlotte Island 26) Pallant Creek; Skeena River 27) Clearwater River; 28) Cedar River; 29) 
Toboggan Creek; 30) Babine River; 34) Sustut River; Nass River 31) Tseax River; 32) Zolzap River; 33) Meziadin River. 
Band software (Millipore Corp. Imaging systems, 
Ann Arbor, MI). The software estimated allele sizes 
to the nearest basepair with a molecular weight-size- 
grid created with a 20-bp DNA ladder. Alleles were 
identified by using a binning procedure (Gill et al., 
1990; Galbraith et al., 1991). Allelic bin intervals 
were created from peaks in allele frequency histo- 
grams and precision estimates from standard test 
fish (Small et al., 1998). Bin intervals for OfslOl and 
Ots 3 alleles are presented in Table 1 and bin inter- 
vals for Ofsl03 alleles are presented in Table 2. The 
mean bin width was four or more standard devia- 
tions from allele sizes of test fish. Allele size was gen- 
erally four bp for OfslOl, two bp for Ots3, and four 
bp for Ohs 103. When alleles became infrequent at 
the extremes of the size ranges and the precision of 
sizing alleles decreased, alleles were grouped into 
larger bins (Small et al., 1998). Thirty-one alleles 
were defined for OfslOl, 20 alleles were defined for 
Ots3, and 37 alleles (as well as a null allele) were 
defined for Ohs 103. 
Data analysis 
Linkage tests confirmed that the three loci are un- 
linked and pedigree analysis confirmed Mendelian 
