868 
Fishery Bulletin 96(4), 1998 
the bloodstream with conventional RIAs (Scott and 
Canario, 1992; Inbaraj et al., 1995). Clearly, addi- 
tional research is needed in this aspect of English 
sole reproductive biology. 
In summary, the reproductive endocrine cycle in 
female and male English sole from two residential 
sites and a spawning site in Puget Sound, WA, was 
characterized by measuring reproductive steroid con- 
centrations and correlating them with collection time 
S O N D J 
Month of collection 
Figure 7 
Mean ± SE of (A) gonadosomatic index (GSI), (B) plasma 
11-ketotestosterone ( 11KT) concentrations, and (C) plasma 
testosterone (T) concentrations in male sole from Tulalip 
Bay, Pilot Point, and University Point at each month of 
fish collection. Line represents mean levels at each month 
of fish collection. Numbers in parentheses represent ani- 
mals sampled each month at each site. 
and histological assessment of gonadal stage. This 
study provides the first description of the reproduc- 
tive endocrine cycle in male English sole and can be 
used as a baseline tool in evaluating the effects of con- 
taminants on reproductive endocrine function in male 
English sole, or in individuals used in mariculture. 
Acknowledgments 
We thank Y. Nagahama, G. Niswender, and A. P. Scott 
for providing antibodies for RIAs, M. J. Willis and T. 
Lee for processing of the histology slides, P. Plesha 
and H. Sanborn for coordinating sampling trips, M. 
S. Myers and C. M. Stehr for reviewing the manu- 
script, and others at Northwest Fisheries Science 
Center for assistance with fishing and necropsy. 
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