*35 
on the Spleen. 
was estimated by adding tincture of rhubarb to alkaline water 
so as to produce corresponding tints. The infusion of spleen 
had a tint equal to sixty drops of tincture of rhubarb in two 
ounces of alkaline water: the serum of the splenic vein to 
fifteen drops : the serum from the left auricle of the heart, 
to three drops. The infusion of the liver gave no orange 
tinge, but had it not been obscured by the red particles of 
the blood, it must have been equal to that of the serum from 
the auricle. 
The connecting membrane between the stomach and spleen 
was attentively examined, very few absorbent vessels were 
seen, and these were not in a turgid state, they were traced 
to the chain of glands situated near the edge of the spleen, 
which receive the absorbents of the stomach, but none were 
detected passing beyond the glands, nor did the glands admit 
quicksilver to pass through them towards the spleen. 
Exp. 2. The former experiment was repeated upon another 
ass, with similar results, but less strongly marked ; the cause 
of this difference was explained by the abdominal viscera being 
in an inflamed state. 
The urine was less impregnated with rhubarb, the infusion 
of the spleen had a lighter tinge, and the serum of the splenic 
vein had it in a still less degree ; but evidently exceeding that 
of the serum from the vena cava inferior opened just below 
the diaphragm, which was substituted for the left auricle of 
the heart, with a view to vary the experiment. 
Exp. 3. The same experiment was made on a third ass 
with similar results. 
Exp. 4. An ass that had been kept four days without water, 
and two without solid food, on the evening of the 8th of 
