NOTE Garcia-Rodriguez et aL: Mitochondrial DNA markers to identify Panulirus spp. 
207 
P. ine-ENSl 
P. inf-JALl 
P.gra-GUE3 
1 
TACAGTTACTTGATTTACTAACACTTGCCAATATACAAATTTCACCTCTTATTGTTTCCAGTACTGAATATAAATG-AACTTAA--TTGTCTAACTTTATAA 
... T ........ . ....A A A.A.TA.AC..AT...C.TT..TT CGGT . CA . T . . . . C . . TAC . 
CT . GCGC . C . A . .TT. . .-.T. . . . . .A T . A A.A.TG. .C. .AT. . .-. .T. .TC CGTT . CA . T . . . . C . . TAT . 
P . ine-ENSl 
P. inf- JAL1 
P.gra-GUE3 
103 
CTTACTCTTTTTCACTGGGCCTTCAAGTCTAACCGCGGATGCTGGCACAAGTTTTAACCTGACCTAAATTGTTTTTACTTAATCCAATCTTTATTGATT-AT 
. . . .T. .ACC A. .G C G.G.A.GC. . .G CA. . .ATC T. . 
. . . .TC.ACC A. .A. A. .G C A.GC. . .G CAT. .AT T. . 
P. ine-ENSl 
P. inf-JALl 
P.gra-GUE3 
205 
TAAATTTTTAGTACTGCTCATGTAAATTATAGCGTTTAGCTTATTATTCGA CTCACTTTAATATTGAATTAAAGTGCCTATATTTTAATGTACT 
A A.C.C... .AAT.GTA. . . C . CAC . -CC.A. . . CGTCGCACC . C . CTC . . CG . . . . T . . CG . . CAGTG . .G. .AA. . . -CAA. . . 
A A.T.TC. . . AAT . . TA . .C.TTAC. . CC . . . TT . - - CACACC . ATTT . CCCG . . . CT . . CG . .C.GTG.C. . .AA. . .-CAA. . . 
P. ine-ENSl 
P. inf-JALl 
P. gra-GUE3 
307 
AATATTTGCATGTACTATTCAAACCAAATAGCGAAAAGAAATAGCAAGAATCAAACTATAGGACCCTACCAACTTATATTTTTATACTTTAATATTGATTTT 
..A T.T G....C. .AT- . . . G . T TA.ATG. .CAA. . . - . G . CC . C . TCGACCC . . . - . .AAA 
..A T.T G AT-...G.T CT . TACA . . CAAGG . - . A . AC . A . T . AATCA . . .T. . .AA 
P. ine-ENSl 
P. inf -JAL1 
P. gra-GUE3 
409 
AATTTTA-ATAGTAGTTTTTTAAGAACTAACACTAATAGATGACCTTTTATACCTATTA- -CCGGCTAAAAGTTTAGTAAAAATACAATCTACTGATATTAT 
.T.A.A.C AC. . .A T.TTTTT. .T. . A . ATGTT A.C. . . A . AA . ACT . ATT . TTC . ACGCCCC . . GTTAC . . . A . GTT . AGCGACTA 
. CCAAATTG . T . . .T.AA.G TATTTTTACT . .A. AT. AT. .AA. . TTTA . AAGATTTAAT . .C--A. . ATCC . GCTTAC . . .A.GCT.A.CG. . GA 
P. ine-ENSl 
P. inf-JALl 
P. gra-GUE3 
511 
TCCCACAGACTTAGAGGACCAAAAAATGGAA GGAAATTTAAAATGCGCCC-ATTAACTAGTATTCGGTTAACAAGTTATTTACCCATTATATATACAT 
CT.G.T.TCG.A.C. . - .AA.TCCG. .TA. . GCCCTA . T . ACC . . - CAATT . AAG . C . . . . CGAC . . . . CAA . TTTTTAC . T . . . -T.T 
C. .G.T.CT. . .CT. . . .AA.TTC. .ATT. . --CCAA.T TCATT . TAAG . C . G . CCTGT . . . CAACC . ATTAC . T . . . - . TTTG 
P. ine-ENSl 
P. inf-JALl 
P . gra-GUE3 
613 
TTAAAGAAAATGTAATTAGACCGTTTCACAATAAGTACAGGGCTT- -ATTTTTTCAACCAATTAAAAATATAAAAAATA-AAGGCTTATACTAATTTAAACA 
. . . . TT . T . - - TA. .A. . . . TCAAG . . . .A. A. . .AA.TAC A. . .T CTG.A.TG. . . CT . .TGA.C.TTG 
. . AAATAAA - TGTAAACAGAC - GTTTCACAA . .TA. .A. .A. TCAAG. . . .A. A. . .AA.TAC T. . . .CC- . . A . TA . . . CT . .TG. .T. . TT 
P. ine-ENSl 
P. inf-JALl 
P. gra-GUE3 
715 
CCATATCTAAAGAGTAACAATTATAGCATGTATTAAACAAGTACGATATGGCTTCCTTTCTAAAAAAGGTTAAATTGGAGATAGATTAGAGCCGATTTTGTA 
TT. . . AGCTCC . . . - A .G. . C . GTG TAGTTCGGTAATGGG - CCATC G - .C. . TTC . C . -G. . . 
,T. . .AA. . .C. . . - . . .TG A . G . ACTGTTT . . GT . .GC.AT.GG- . .C.C.C. . . . TTC . C . .G. . .T GAC . .T 
817 
P . ine- ENS1 CTTTACCCCCAGGAAGTACTGTAAAATGCGGCTCTAATCTATCCACATATACTAATGCAAGCCTTTATAAACCATTTCAGCGAATATAACTTATTATTAGAT 
P. inf-JALl 
P . gra-GUE3 
919 
P. ine-ENSl TATACACCTTATTGTATGTTTAATAAAACTTAAATGTATATATATCTAGAGAT ATCCATTAGCCTGAATTTTT AGCCCCTCCCCCCCCTTTCTCATCCTCTA 
P. inf-JALl 
P. gra-GUE3 
1021 
P . ine- ENS 1 TCTGAGTTTATTTCTTAAATTAATACAATTTTATATTTGGCTGGCGTGGTTAGTTTATATTTAATTAAATATTTTGCCATTAAACATGATGCCTGATAAAAG 
P. inf-JALl 
P. gra-GUE3 
1123 
P. ine-ENSl GTTTGTTTTGATAGGACAAGTAATGCAGATAAAACTGCTCATGTTACAAGTAACGGGATCACACCAATTCCTAGAATATCAAAAATCTTATGCTC 
P. inf-JALl 
P. gra -GUE3 
Figure 1 
Alignment of control region sequences of California spiny lobster (Panulirus interruptus [ P. ine ]), blue spiny lobster (P. 
inflatus [ P.inf]), and green spiny lobster (P. gracilis [ P.gra ]) used to find specific primers from one specimen of each 
lobster species. Underlined regions indicate species-specific primers. Label after the species name indicates specimen’s 
voucher code. Numbers 1, 103, 205,..., 1123, indicate positions in the sequence of the first nucleotide in the row. 
ilis (Table 2). Thus the composite haplotypes are able 
to delineate among these species. All electrophoretic 
patterns of PCR-RFLP products of adult specimens 
were congruent with those obtained from sequences 
analysis. Finally, the sequence of P. penicillatus had 
restriction sites that would produce different patterns 
from the other species (Fig. 3). 
Multiplex PCR 
A high annealing temperature (59°C) was adequate for 
successful multiplex PCR reactions containing the DNA 
of the three species. There were no additional fragments 
to those expected that could prevent identification of 
lobster species in all tested adult specimens. The size of 
