132 
BULLETIN OF THE UNITED STATES FISH COMMISSION. 
phosphoric acid and sulphuric acid. The solid residue (h) from the HC1 extract was 
washed with water and extracted several times with hot chloroform, which took out 
small quantities of bilirubin at each extraction ( i ). This method evidently did not 
remove all the pigment, so the solid residue (j) was extracted with hot alcohol 
containing a little HC1. This removed a pigment in considerable quantities (k) 
which gave the qualitative tests for bilirubin. The solid residue (?) was digested in 
ether for 12 hours, but nothing was extracted (m). The residue (n) gave strong- 
reactions with M i 1 Loti’s reagent and with the xanthoproteic test. It was divided into 
two proportions. One portion (o) was oxidized with KNO., and Na 3 C0 3 and tested 
for phosphoric and sulphuric acids. Both were positive. A control test with the 
reagents alone gave no reaction. The other portion (p) was boiled with dilute hydro- 
chloric acid for two hours. The resulting solution was examined for reducing sugars, 
but with negative results. It would seem, then, from the above that the proteid 
substance was of the nature of a nucleo-albumin and not a mucin. (See Table I.) 
The small amount of calculi in the first or upper group (plate xxi) not used in 
the preceding analysis was estimated quantitatively for the principal constituents, 
with the following results: 
Calculi dried to constant weight over H 2 S0 4 . 
Per cent. 
Cholesterin and fat 2. 85 
Mineral 3. 65 
Bilirubin 16. 14 
N ucl eo-albumin 65. 59 
Water 
Soluble in water 
Total ash of calculi 
The fat was in excess of the cholesterin, although a quantitative separation was 
not made. 
The calculi in the second group (plate xxi) were from another squeteague (a 
male). They differed from the first lot in being smoother and less nodulated. When 
bisected longitudinally they exhibited the same concentric structure as the others. 
These stones were found not only in the gall bladder and gall ducts, but also in the 
intestines, and, strangely enough, were embedded in the tissues between the liver and 
intestines. No lesions or scars appeared either in the bile ducts or in the intestines, 
vet these stones must have broken through the walls of these passages at some earlier 
time. The gall bladder and duct contained a small quantity of bile. 
There were in all eleven stones, weighing 1.865 grams. The largest dried over 
sulphuric acid weighed 0. 615 grams, was 1.7 cm. long, 0.8 cm. wide, and 0.7 cm. thick. 
The qualitative analysis agreed with the preceding and was carried out in the following 
way: The dried substance, finely minced, was extracted with ether until nothing more 
was dissolved. The ether extract (1) on evaporation contained cholesterin and a 
small amount of fat (see p. 135). The residue (2) was digested with dilute (2 per cent) 
hydrochloric acid for 12 hours, giving a slight effervescence of C0 2 . The extract 
(3) on evaporation showed but little residue and was united with (5) for further 
analysis. The residue (1) was now extracted four hours with warm dilute HC1, 
which removed most of the mineral matter. The extract (5) was analyzed directly 
for inorganic substances. This gave relatively large quantities of phosphoric acid, 
Trace. 
99. 75 
4.32 
