134 
BULLETIN OF THE UNITED STATES FISH COMMISSION. 
Table I. 
The finely minced calculi boiled 
with water and filtered. 
Water extract (a). Traces of or- 
ganic matter. 
Residue (b) extracted with hot 
alcohol. 
Alcohol extract (c). Traces of 
cholesterin and pigment. 
Residue (cl) extracted with ether. 
Ether 
and fat. 
extract (e). Cholesterin 
Acid solution ( g ). Evaporated, 
ignited. Ash contained Ca, Mg, Pe, 
H 3 P04, H0SO4, Iv, and Na. 
Residue (/) extracted with dilute 
HC1 (1:3). Effervescence of C0 2 . 
Residue ( h ) extracted with hot 
chloroform. 
Chloroform extract ( i ). 
amount of bilirubin. 
Small 
Residue ( j ) extracted with hot 
alcohol containing dilute HC1. 
Acid-alcohol extract (fc). Bili- 
rubin. 
Residue ( l ) extracted with ether, 
12 hours. 
Ether extract (to) evaporated. No 
residue. 
Residue (n) divided into two 
parts. 
Part (0) boiled 2 hours with dilute 
HC1. No reducing substance. No 
mucin. 
Part (p) oxidized with KNO ;l and 
Na«C0 3 . Dissolved. Tests for H3PO4 
and HoS 0 4 . Nucleo-albumin. 
