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One of the insecticides developed by Eiensschneider and Kuhnl, called 
M-410, is stated to have the same structural formula as that assigned to 
chlordane. No details are given regarding its synthesis or action on 
insects but it is claimed to be no more toxic than DDT to mice. 
ANALYSIS 
There are no specific chemical and physical methods for determining 
chlordane. 
Ard (_22) has described a qualitative test for chlordane in insecti- 
cide spray oils. The method consists in mixing 1 ml. of the sample in a 
test tube with 2 ml. of a Cello solve-pyridine (^+0:10) solution and 1 ml. 
of an approximately 1 N solution of potassium hydroxide in 95 percent 
ethyl alcohol. When heated in a boiling water bath with occasional agi- 
tation for 5 minutes, the appearance of a red color indicates the presence 
of chlordane. A 1-ml. sample of 0.2$ chlordane in a deodorized kerosene 
base gives a wine-red color of considerable strength, and 1 percent gives 
an intense dark red color. Very weak colors should be regarded as pos- 
sibly due to other substances. A confirmatory test for chlordane it the 
appearance of an odor resembling that of a crude methylnaphthalene when 
the sample is dehalogenated with sodium in boiling isopropyl alcohol as 
is done in the determination of total chlorine. Toxaphene is the only 
other insecticide that yields a similar odor when subjected to this test. 
Some chemists using Ard 1 s method have pointed out that the red color 
developed is due to by-products in the technical grade of chlordane and 
that pure chlordane does not give this color. The method would appear to 
be applicable, however, if the amount of col or -producing materials were 
a constant in the commercial product. 
Alessandrini (iP_) modified Ard 1 s method by substituting ethylene 
glycol for Cellosolve (the monoethyl ether of ethylene glycol). The 
coloration produced is less intense than in the original method. The 
presence of DDT does not interfere with the coloration. 
Analyses of chlordane formulations by this method and by the deter- 
mination of total and labile chlorine have been published by Alessandrini 
and Amormino (ll) . 
