- 17 - 
more severe for those treated with chlordane. The time lapse "between 
administration of an acute lethal dose and death is longer for chlordane 
than for DDT treated rats. There is some indication that chlordane may 
he slightly more toxic to female rats than to males. This was not ob- 
served to he true for DDT- treated rats. Chlordane appears to produce in 
rats less liver damage, hut greater pulmonary damage than DDT. — Ingle 
(235). 
Pharmacologists of the Food and Drug Administration found that the 
acute oral toxicity of chlordane to rats was LD-50 500 mg./kg. t indicating 
that it is 1/2 as toxic as DDT. Chlordane is quite toxic when fed to rats 
at a concentration of 250 p.p.m. for 12 weeks. Rats have "been seriously 
injured at this level and fail to survive on higher concentrations. 
Chlordane is a liver poison and also causes inanition in chronically 
poisoned animals. — Lehman ( 287 . 290 .) . 
Chlordane is absorbed by the animal but its fate and whether or not 
the chemical is excreted in the urine is unknown. — Woodward et al. ( 509 ) . 
No harm resulted to rats, mice, or guinea pigs when they were sub- 
jected for 45 minutes on each of 38 days to air bearing aerosols contain- 
ing chlordane with methylene dichloride or dimethyl phthalate as solvents, 
the average initial concentration of chlordane being approximately 1.9 mg. 
per liter. When aerosols with either kerosene, methylene dichloride, or 
dimethyl phthalate as a solvent were introduced into the chamber at inter- 
vals of 10 minutes over an hour in an initial concentration of 10 mg. of 
chlordane per liter, and this procedure was repeated three times on each 
of four successive days, many of the animals exhibited typical signs of 
poisoning by chlordane. — Heyroth and Witherup ( 217 ) • 
Stohlman et a^. (V+2, 443 ) reported that the LD-50 of chlordane 
when fed to rats is 250 mg./kg. compared with 150 mg./kg. for DDT. 
Rabbits receiving chlordane excrete organically bound chlorine in the 
urine. The LD-50 of chlordane when administered intraveneously to rab- 
bits is about 20 mg./kg. and this is increased to about 60 mg./kg. 
through the antidotal action of the barbiturates. 
Frings and 0'Tousa ( 153 ) reported that chlordane is very similar to 
DDT in its toxic action in mice. The first system affected in the nervous 
system, and nervous symptoms predominate in acute toxicity. In chronic 
intoxication, however, the liver seems to be most affected. The vapor of 
chlordane was toxic and wettable powder preparations proved surprisingly 
toxic. 
(b) Dogs - Dogs starved for Ik hours were fed a 50-percent chlordane 
wettable powder in capsules with oil. It was concluded that chlordane, 
like benzene hexachloride, is a relatively safe insecticide to use upon 
dogs. Dogs vary in their susceptibility to chlordane. A dose of 200 mg./ 
kg. produced convulsions in one dog, while a 700 mg./kg. dose had little 
effect on another dog. — Batte and Turk (29,) . 
