February 1951 E-813 
United States Department of Agriculture 
Agricultural Research Administration 
Bureau of Entomology and Plant Quarantine 
EQ 335 AND OTHER WOUND TREATMENTS FOR 
SCREW-WORM CONTROL 
Jl 
Prepared by the Division of Insects Affecting Man and Animal 
A new smear has been developed as a wound treatment for controlling 
the screw-worm on livestock and fleeceworms, or wool maggots, on 
sheep. This new treatment, recommended as a supplement to older 
treatments known as smear 62 and EQ smear 82, is designated as 
"EQ 335 screw-worm remedy." The figure "335" represents the con- 
centrations of the two main active ingredients, lindane (3 percent) and 
pine oil (35 percent). 
EQ 335 does not stain, is not highly volatile, and has proved more 
effective in preventing screw-worm infestations than either smear 62 
or EQ smear 82. Most important, however, is the fact that EQ 335 will 
kill screw-worm flies visiting wounds that have been treated with it. 
While the EQ 335 screw-worm remedy was being developed, several 
other preparations containing lindane and pine oil proved satisfactory as 
screw- worm smears. The composition, methods of preparation, and 
use of two of them, and also use of a liquid formula containing these 
materials, are discussed briefly. 
EQ 335 SCREW-WORM REMEDY 
Composition and Preparation 
Percent by Weight 
Lindane 3 
Pine oil 35 
Mineral oil 42 ±2 
Emulsifier 10±2 
Silica aerogel 10i2 
1/ This treatment was developed by Gaines W. Eddy with the assist- 
ance of W. S. McGregor, D. E. Hopkins, J. M. Dreiss, and James 
Cairnes. Investigations on the toxicity of lindane preparations on live- 
stock were conducted by R. D. Radeleff and W. G. Nickerson, Bureau 
of Animal Industry. 
