2 - 
A number of fractionated pine oils have been used. Satisfactory 
results were obtained with water-white or light-colored pine oils, specific 
gravity 0.92-0.94, with distillation range of 198°-225° C. Materials used 
in development of the preparation were obtained from the Hercules Powder 
Company and Newport Industries. 
The mineral oil is a technical-grade white oil of 80-90 seconds 
Saybolt viscosity at 100° F. 
The following emulsifiers have proved satisfactory: Trex 80 and 
Tenlo 400 (Griffin Chemical Company); Petromix #9 (L. Sonneborn Sons, 
Inc.); Ahcowet RS, anhydrous (Arnold Hoffman & Company); Petrobase 1, 
grades A, B, or C, and Petrobase 2, grades B or C (Pennsylvania Refining 
Company); Atlox 1045a or equal parts of Span 80 and Tween 80 (Atlas 
Powder Company). 
The silica aerogel used in these tests is the product called Santocel C 
(Monsanto Chemical Company). In previous releases on the EQ-335 
remedy this material was erroneously called silica gel. 
The lindane may be dissolved either in the pine oil or in a mixture of 
the pine oil, mineral oil, and emulsifier. The use of gentle heat is re- 
commended. The silica aerogel may be added to the lindane solution or 
the solution added to the silica aerogel, whichever proves the most con- 
venient. The mixture should be stirred until a smooth smear is obtained. 
It is then ready for use. 
Functions of the Various Ingredients 
Lindane . --Lindane is the main active ingredient, being the essential 
larvicide and wound protector in the formula. 
Pine Oil. --Pine oil is used mainly as a solvent. However, it also 
acts as a larvicide, and is therefore considered an active ingredient. 
Alone, at the concentration used, it will cause complete mortality of 
young ( 1 - to 2-day) screw-worm larvae. It is irritating to screw-worms 
and especially fleeceworms, causing them to move about, thereby coming 
into more intimate contact with the lindane. Pine oil is also reputed U 
be a good bactericide. 
Other ingredients. --Mineral oil is used as a diluent, mainly because 
of its freedom from irritating effects on the skin and wounds of animals. 
It reduces the irritating effect of the high concentration of pine oil. 
Silica aerogel is used as a thicking agent. An emulsifier is used so that 
the material will mix with the wound fluids arid also with water for use 
linsl fleeceworms. 
