OF INDIGO IN HUMAN URINE. 
299 
urine in this sample became thick and of a dirty grass-green colour, and a similar 
brown deposit with some blue colouring matter was found at the bottom. Some of 
the blue and brown deposits from this and the previous sample were collected and 
subjected to analysis. 
The third, fourth and fifth samples resembled very closely the second, but con- 
tained less of the blue and brown colouring matters. 
The sixth sample was neutral, of specific gravity 1007, and contained rather much 
vaginal epithelium. A pellicle gradually formed, limited principally to the borders 
of the fluid ; this became of a decided bluish tint, the urine itself at the same time 
becoming dark brown, letting fall a deposit of the same colour, and possessing 
the same characters as in the other samples. Examined with the microscope, pieces 
of the blue colouring matter were detected in the scum, as well as triple phosphate, 
Vibriones, and a few of the animalcules. 
The seventh urine was decidedly alkaline, of specific gravity 1019, and contained 
much mucus and epithelium ; eight days afterwards, a scum, which had become deep 
blue , had formed around the margin of the fluid ; the urine had changed from light 
brown to a dark greenish tint, and was very thick, some of the brown sediment 
present in the other samples being found at the bottom of the glass. 
The eighth urine was of a straw-colour, slightly acid, of specific gravity 1015, and 
contained much mucus and epithelium; at the end of eight days, the scum, which 
had formed chiefly around the edge of the fluid, was perceptibly blue on one side only, 
but the colour was neither so deep nor so extensive as in the previous sample. The 
urine itself became dark and thick. 
The ninth sample was decidedly acid, of specific gravity 1009, and contained 
albumen, many blood-corpuscles, and much mucus. The pellicle which formed around 
the edge of the fluid did not become in the least blue, neither did the urine undergo 
any very considerable change of colour, although it became a few shades deeper. 
The tenth sample was slightly acid, also of specific gravity 1009, and contained 
much epithelium, but no albumen. A thick scum of Vibriones and animalcules 
collected after a time over the entire surface, but it did not become at all blue ; the 
urine was only a few shades deeper coloured than when passed. No blue particles 
were discovered with the microscope. 
I will now proceed to state the results obtained by the chemical examination of the 
urine, the blue colouring matter, and the brown extractive, as made by Dr. Letheby 
and myself. The analysis of the urine was made after it had been standing for some 
time, and after it had been shaken and disturbed, its appearance being considerably 
altered thereby. 
The Urine . — The urine of the second sample at the time of analysis, when shaken 
up, had a dark greenish-brown colour, was strongly alkaline both from fixed and 
volatile alkalies, and emitted a highly ammoniacal odour. It was turbid from the 
presence of a large quantity of triple phosphate, as also from the colouring matter; 
2 Q 2 
