214 BUREAU OF ENTOiVrOLOGY AND PLANT QUARANTINE [July-Sopt. 
of the Mediterranean frnit fly in fruit will be destroyed if the fruit is subjected 
to the following treatment : 
Cooling until the approximate center of the fruit in the package reaches a 
temperature of 34° F. and holding the fruit at or beiow that temperature for a 
period of 12 days. 
On the basis of the evidence obtained and under ihe authority of regulation 
2 of the rules and regulations supplemental to Notice of Quarantine No. 56, 
provision is hereby made for the entry, under permit, and sterilization at plants 
designated for the purpose, of grapes of the Vinijiera type from regions in which 
the Mediterranean fruit fly occurs, at the port of New York and such other 
northern ports as may be subsequently approved, under the following conditions : 
1. The grapes must be packed in tight barrels or kegs or other approved 
containers so constructed as to prevent the escape from the containers pending 
sterilization, of any stages of the Mediterranean fruit fly, should they be present. 
Unsterilized grapes in broken containers must be immediately repacked under 
the supervision of an inspector of the Bureau of Entomology and Plant Quaran- 
tine or the contents shall be immediately destroyed in a manner satisfactory 
to the inspector. 
2. Within 24 hours from the time of unlading, the grapes shall be delivered for 
treatment to a designated cold-storage plant. 
The Bureau of Entomology and Plant Quarc.ntiue will designate only those 
cold-storage plants which are adequately equipped to handle and sterilize the 
grapes. An application and a written agreement in form prescribed must be 
filed with the Bureau of Entomology and Plant Quarantine as a condition for 
designation. 
The sterilization of grapes and their movement to and from the sterilization 
rooms shall be done under the supervision of plant quarantine inspectors of 
the Bureau of Entomology and Plant Quarantine who shall at all times have 
access to the grapes. 
Shipments offered for entry may be allowed to leave customs custody under 
redelivery^ bond for sterilization. Final release of the shipment by the collector 
of customs and cancelation of the bond will be effected after the inspector of 
the Bureau of Entomology and Plant Quarantine has notified the collector of 
customs that the required treatment has been given. 
3. For the purpose of additional safeguards and to eliminate possible risk 
that might be occasioned by breakage of containers, the entry of grapes is 
limited to the period from October 1 to April 15, when susceptible fruits will 
not be available for oviposition by fruit flies should any escape prior to the 
containers being placed in the approved sterilization chambers. 
In authorizing the entry of Vinifera grapes into the United States subject 
to sterilization in accordance with the provisions of this circular it should be 
emphasized that inexactness and carelessness in applying the treatment may 
result in injury to the grapes. 
The treatment required for the entry of Vinifera grapes under the provisions 
of this circular represents a requirement considered necessary to eliminate 
pest risks and no liability shall attach to the United States Department of 
Agriculture or to any officer or representative of that Department in the event 
of injury resulting to fruit offered for entry under the provisions of this 
circular. 
Circular B. E. P. Q. 417 is hereby revoked. 
Lee A. Strong, 
Chief, Bureau of Entomology and Plant Quarantine. 
B. E. P. Q — 404 
EXPORTATION OF VINIFERA GRAPES AND CERTAIN OTHER DECIDUOUS FRUITS 
SUBJECT TO IN-TRANSIT STERILIZATION AUTHORIZED 
[Approved and effective September 15, 1937] 
It has been determined that the refrigeration treatment prescribed in cir- 
cular B. E. P. Q. 403. as a condition for the entry of Vinifera grapes from 
regions in which the Mcditorranean fruit fly occurs, can be completed while 
the fruit is in transit on ships equipped with adequate refrigeration facilities, 
provided the grapes have been cooled to the proper temperature before loading 
in refrigerated bolds in the carrying vessels. It has also been determined that 
certain other deciduous fruits can be similarly treated. 
