70 BUREAU OF ENTOMOLOGY AND PLANT QUARANTINE [July-Sept. 
ANNOUNCEMENTS RELATING TO JAPANESE BEETLE QUARANTINE 
(NO. 48) 
B. E. P. Q. 499, 
Supplement No. 1 — Fourth revision. August 6, 1941 
TITLE 7— AGRICULTURE 
Chapter III — Bureau of Entomology and Plant Quarantine 
PART 301 DOMESTIC QUARANTINE NOTICES 
JAPANESE BEETLE ADMINISTRATIVE INSTRUCTIONS MODIFIED 
INTRODUCTORY NOTE 
It has been found as a result of additional experiments with treatments 
for freeing nursery stock and potted plants from the immature stages of the 
Japanese beetle that the methyl bromide fumigation treatments may be further 
modified. Accordingly these instructions provide for a temperature of 67° F. 
instead of 70° as heretofore, for the 2% -hour treatment with a 2-pound dosage; 
and for 73° under a 2%-hour treatment with a iy2-pound dosage. The diameter 
of the soil balls in all treatments is placed at a maximum of 14 inches instead of 
12 inches as heretofore. 
§ 301.48-b. Administrative instructions to inspectors on the treatment of nurs- 
ery products, fruits, vegetables, and soil, for the Japanese beetle. Treatment 
authorized. Pursuant to the authority conferred upon the Chief of the Bureau 
of Entomology and Plant Quarantine by § 301.48-6, Chapter III, Title 7, Code of 
Federal Regulations [regulation 6 of the rules and regulations supplemental 
to Notice of Quarantine No. 48] subsection (1) (5) of § 301.48b 1 [on page 13 
of the mimeographed edition of circular B. E. P. Q. 499, issued June 9, 1939] 
is hereby further modified effective August 9, 1941, to read as follows : 
(5) Methyl bromide fumigation 
Equipment. — An approved fumigation chamber equipped with vaporizing, air- 
circulating, and ventilating systems must be provided. 
Application. — After the chamber is loaded, the methyl bromide must be va- 
porized within it. The air within the chamber must be kept in circulation during 
the period of fumigation. At the completion of the treatment, the chamber must 
be well ventilated before it is entered and the plants removed. The ventilating 
system should also be in continuous operation during the entire period of removal 
of the fumigated articles. 
(i) Fumigation of plants, with or without soil 
Temperatures, periods of treatment, and dosages. — The temperature of the soil 
(with bare root stock, the root spaces) and of the air for each type of treat- 
ment must remain throughout the entire period of treatment at the minimum 
specified in the following table, or higher : 
Temperature at least 
Period of 
treatment 
Dosage 
(methyl 
bromide 
per 1,000 
cubic feet) 
Temperature at least 
Period of 
treatment 
Dosage 
(methyl 
bromide 
per 1,000 
cubic feet) 
1. 73° F 
Hours 
2V 2 
2V 2 
2V 2 
3 
Pounds 
2 
5. 57° F 
Hours 
4 
Pounds 
2V 2 
2V 2 
2Vz 
2. 67° F 
6. 54° F 
3. 63° F 
2V 2 
2Yi 
7. 50° F 
4. 60° F 
The dosage shall be for each 1,000 cubic feet including the space occupied by 
the load. 
1 This section was originally issued as § 301.48a. 
