46 BUREAU OF ENTOMOLOGY AND PLANT QUARANTINE [April-June 
B. E. P. Q. 499, Supplement No. 1, Third Revision. 
April 11, 1941. 
TITLE 7— AGRICULTURE 
Chapter III — Bureau of Entomology and Plant Quarantine 
Part 301 — Domestic Quarantine Notices 
japanese beetle administrative instructions modified 
INTRODUCTORY NOTE 
It has been found as a result of further experiments with treatments for freeing 
nursery stock and potted plants from the immature stages of the Japanese beetle, 
that 2y 2 pounds of methyl bromide per 1,000 cubic feet applied for a period of 
3% hours at a temperature of not less than 57° F., or for 3 hours at not less 
than 60° F., is sufficient to kill such larvae. These instructions are accordingly 
revised to authorize the use of such treatments in addition to the four schedules 
heretofore provided for in the second revision of supplement No. 1 to this circular. 
§ 301.48-b Administrative instructions to inspectors on the treatment of nursery 
products, fruits, vegetables, and soil, for the Japanese beetle. Treatment author- 
ized. Pursuant to the authority conferred upon the Chief of the Bureau of 
Entomology and Plant Quarantine by § 301.48-6, Chapter III, Title 7, Code of 
Federal Regulations [regulation 6 of the rules and regulations supplemental to 
Notice of Quarantine No. 48] subsection (1) (5) of § 301.48b 1 [on page 13 of the 
mimeographed edition of circular B. E. P. Q. 499, issued June 9, 1939] is hereby 
further modified to read as follows : 
(5) Methyl bromide fumigation 
Equipment — An approved fumigation chamber equipped with vaporizing, air- 
circulating, and ventilating systems must be provided. 
Application, — After the chamber is loaded, the methyl bromide must be vapor- 
ized within it. The air within the chamber must be kept in circulation during 
the period of fumigation. At the completion of the treatment, the chamber 
must be well ventilated before it is entered and the plants removed. The ven- 
tilating system should also be in continuous operation during the entire period 
Of removal of the fumigated articles. 
(i) Fumigation of plants, with or without soil 
Temperatures, periods of treatment, and dosages. — The temperature of the soil 
(with bare root stock, the root spaces) and of the air for each type of treatment 
must remain throughout the entire period of treatment at the minimum specified 
in the following table, or higher: 
Temperature at least 
Period of 
treatment 
Dosage 
(methyl 
bromide 
per 1,000 
cubic feet) 
Temperature at least 
Period of 
treatment 
Dosage 
( methyl 
bromide 
per 1,000 
cubic feet 'i 
1. 
70° F_ H1 
Hours 
2V* 
3 
Pounds 
2 
4. 57° F 
Hours 
m 
4 
Pounds 
VA 
2. 
63° F 
2V2 
5. 54° F 
3. 
60° F 
6. 50° F 
4H 
The dosage shall be for each 1.000 cubic feet including the space occupied 
by the load. 
Preparation of plants. — The treatment is to be applied to plants with bare 
roots or in 12-inch pots or smaller, or in soil balls not larger than 12 inches in 
diameter nor thicker than 12 inches when not spherical. The soil should not 
1 This section was originally issued as § 301.48a. 
