26 BUREAU OF ENTOMOLOGY AND PLANT QUARANTINE [Jan.-March 
been applied, the soil balls shall remain embedded in the sand for a period of 
8 hours. The temperature of the soil balls during the treatment shall not be 
lower than 65° F. 
(ii) In all regulated areas other than Orleans Parish and Saint Bernard 
Parish, La. After the required dosage has been applied, the soil balls shall 
remain embedded in the sand for a period of 8 hours. The temperature of the 
soil balls during the treatment shall not be lower than 55° F. 
(d) Disclaimer. There has been opportunity to test this treatment on only 
relatively few varieties of plants, and it is understood that no liability shall attach 
either to the United States Department of Agriculture or its employees in the 
event of injury to either plants or operators. 
(e) Caution. Methyl bromide is a gas at ordinary temperatures. It is color- 
less and practically odorless, and in preparing the solution the operator should 
wear an approved gas mask. 
This revision supersedes all previous issues of circular B. E. P. Q. 503. 
Avery S. Hoyt, 
Acting Chief, Bureau of Entomology and Plant Quarantine. 
[Filed with the Division of the Federal Register February 28, 1941, 11 : 36 a. m. ; 
6 F. R. 1207.] 
B. E. P. Q. 486, revised. March 21, 1941 
TITLE 7— AGRICULTURE 
Chapter III — Bureau of Entomology and Plant Quarantine 
Part 301 — Domestic Quarantine Notices 
white-fringed beetle regulations modified 
§ 301.72-5b Administrative instructions modifying the restrictions of the 
white-fringed beetle quarantine by authorizing alternative treatments by methyl 
bromide fumigation of plants in pots or in soil halls. Introductory note. — The 
instructions in circular B. E. P. Q. 486, issued January 28. 1939. provided for 
treatment of balled and burlapped nursery stock by methyl bromide fumigation 
for larvae of the white-fringed beetle and limited the treatment to plants in 
3-inch pots or in 3-inch soil balls, and provided for an exposure of 4 hours 
with a dosage of 1 pound of methyl bromide per 1.000 cubic feet at atmospheric 
pressures and a temperature of not less than 85° F. As a result of more 
recent investigations, the instructions are now revised to provide also that soil 
balls not greater than 11 inches in diameter, nor thicker than 11 inches if 
not spherical, may be fumigated under partial vacuum, equivalent to at least 
24.5 inches of mercury, with a dosage of 4 pounds of methyl bromide per 
1,000 cubic feet of chamber space, with an exposure of 1% hours, at a minimum 
temperature of 75° F. These treatments are applicable to both species of the 
white-fringed beetle, Pantomorus leucoloma Boh. and P. peregrin us Buch. 
(a) Treatments authorized. — Pursuant to the authority conferred upon the 
Chief of the Bureau of Entomology and Plant Quarantine by subsection (a) 
of § 301.72-5, Chapter III. Title 7, Code of Federal Regulations (Regulation 5 
of Notice of Quarantine No. 72 on account of the white-fringed beetle), the 
following methods of treatment for certain kinds and sizes of potted and 
balled and burlapped plants are hereby authorized, when carried out under 
the supervision of an authorized inspector of the United States Department 
of Agriculture. 
(b) Fumigation treatment at atmospheric pressures. — (1) Fumigation must 
be done with methyl bromide at a dosage of 1 pound per 1,000 cubic feet, 
including the space occupied by the plants, for a period of 4 hours, the soil 
masses and the air in the fumigation chamber to be at a temperature of not 
less than 85° F. 
(2) Such fumigation shall apply only to those plants in 3-inch pots or 
smaller, or in soil balls not larger than those which would be contained in 
3-inch pots, and the plants shall be stacked on racks so that the gas mixture 
can have access to all* sides of the pots or the soil balls. 
(3) The fumigation shall be done in a tight chamber with gas-tight doors. 
(4) After the chamber is loaded and closed, the appropriate amount of 
methyl bromide shall be volatilized therein, and the air-gas mixture shall be 
