80 BUREAU OF PLANT QUARANTINE 
Uuly-Sept. 
For microscopic examination, tease out the material of the discolored area in 
a few drops of water on a glass plate and examine as described for field inspection. 
If the local inspector has not facilities for such examination, the suspected bulb 
should be submitted to the State inspector and through him to a specialist for 
definite determination. Unless some other special provision is made in the State 
concerned, suspected bulbs may be sent to the Office of Domestic Plant Quaran- 
tines, Bureau of Plant Quarantine, Washington, D. C, for diagnosis. So far as 
practicable, a specialist should confirm the diagnosis in the case of at least one 
bulb from each infested planting. 
Extent of inspection. — Bulbs of every variety produced by the grower are to be 
examined in the warehouse. If a careful and complete field inspection has been 
made, if no eelworms are known to occur on the premises of the grower concerned, 
and if there is no history of eelworm infestation on the premises, a careful exami- 
nation of 10 per cent of the bulbs of each varkty may be sufficient; otherwise, 
at least 25 per cent of each variety should be examined. It is, of course, unneces- 
sary to complete the full 10 per cent or 25 per cent after positive evidence of 
eelworm infestation has been found. 
As fully outlined in B. P. Q. — 337, the grower's premises constitute the unit 
with respect to bulb-fly infestation, but the blocks and varieties may be considered 
separately as to eelworm infestation. See that circular for details. 
When may permits be issued on the basis of storage inspection without field inspec- 
tion? — In the case of bulbs forced under glass, the blossoming period is extended 
throughout the winter by forcing different lots from time to time. This con- 
dition often makes an inspection of the growing plants impracticable. Inspectors 
are accordingly authorized to confine their inspection of forced narcissus to the 
dormant bulbs, and to certify them as free from infestation if no narcissus bulb 
flies or eelworms are discovered. Such inspections must be made with especial 
care as bulbs of this class may become infested while in the florist's possession, 
and if then returned to the grower untreated, the latter's premises may become 
infested. Infested greenhouse-grown bulbs removed from the soil in late winter 
and held until summer before treatment will need to be presoaked, as described 
in Circular B. P. Q — 337. 
Field-grown bulbs that have not received the growing-season inspection are 
to be treated before permits are issued. In special cases, however, the chief of 
the Bureau of Plant Quarantine may authorize the issuance of permits for bulbs, 
on the basis of dormant inspection only, on receipt of evidence that the inspector 
concerned has shown unusual ability in detecting slight eelworm infestations in 
dormant narcissus and that a particularly complete and intensive inspection of 
the dormant bulbs has shown freedom from infestation. 
No tolerance permitted. — The narcissus-bulb quarantine is established, not for 
the maintenance of the "grade and quality" of the bulbs, but to prevent the 
spread of infestation. No tolerance of known infestation, however slight, can 
be authorized as to bulbs moved under Federal permits. 
RECORDS AND REPORTS 
Permit forms. — When the inspector determines to his own satisfaction that the 
narcissus plantings of any grower are free from infestation and that all necessary 
treatment, suppression, and sanitation measures have been carried out, he may 
issue to the grower a certificate of freedom from infestation (Form 388) or a 
certificate of disinfection (Form 389) and such number of shipping permits 
(Form 391) as may be required for the movement of the crop concerned. The 
shipping permits must in every case be made out in full before delivery to the 
grower or shipper. 
Complete the treatment before starting to issue permits. — The maintenance of the 
identity of the bulbs in the case of large plantings where fumigation and treatment 
occupy from several days to several weeks, is a special problem which must be 
worked out by the inspector as to each grower. In general, unless authorized 
by the chief inspector cf the State, no shipping permits are to be issued until 
after all bulbs to be dug that season have been taken up and inspected, until all 
necessary fumigation and treatment for the narcissus on the premises has been 
completed for the season, and until all culls have been properly destroyed. In 
the case of large plantings, the practice of starting to issue permits before all 
bulbs are dug and all treatment is completed has in the past resulted in mixing 
treated with untreated material, the shipment of infested bulbs, uncertainty, and 
in some cases fraud. 
