1934] 
SERVICE AND REGULATORY ANNOUNCEMENTS 
35 
asperma, B. vulgaris atropurpurva, B. vulgaris emarginata, B. vulgaris fruo- 
toviolacca, B. vulgaris japonicu, B. vulgaris lutea, B. vulgaris macrocarpa, B. 
vulgaris mitis,B. vulgaris nigra, B. vulgaris purpurea, B. vulgaris sanguinolenta, 
B. vulgaris spathulata, B. v-ulgaris sheyalle, B. vulgaris sulcata, B. vulgaris 
violacea, B. wilsonae, and B. xanthoxylon (knightii). 
D. SPECIES OB VARIETIES OF BERBERIS OR MAHONIA FOR WHICH REACTION TO 
BLACK STEM -RUST ATTACK HAS NOT BEEN DETERMINED 
Interstate shipments of the following species or varieties must not be made 
into the protected States or from any protected State to any other protected 
State. Permits will not be issued for such movement this season pending final 
determination of the reaction of such species or varieties to black stem-rust 
attack. 
Berberis acicularis, B. buxifolia pygmea, B. californica, B. dictyophylla, B. 
dulcis (buxifolia) , B. lienryana, B. heteropoda, B. hookeri, B. insignis, B. parvi- 
folia, B. pinnata = fascicularis (Mahonia), B. thunbergii x juUanae (hybrid), 
B. tischleri, B. virescens, B. ivilsonac Autumn Cheer, B. wilsonae Fireflame, 
B. wilsonae Firefly, and B. wilsonae Sparkler. 
ANNOUNCEMENT RELATING TO FRUIT AND VEGETABLE 
QUARANTINE (NO. 56) 
B.P.Q.-362. 
STERILIZATION OF IMPORTED VINIFERA GRAPES BY REFRIGERATION 
(Approved Apr. 19, 1934; effective May 1, 1934) 
Regulation 6 of the Fruit and Vegetable Quarantine (Quarantine No. 56), 
as amended effective August 1, 1933, reads in part, as follows : 
"All importations of fruits and vegetables shall be subject as a condition 
of entry to such inspection or disinfection, or both, as shall be required by 
the inspector of the Department of Agriculture." 
Recent experimental work by the Bureau of Entomology of the United 
States Department of Agriculture has proved that all stages of the Mediter- 
ranean fruit fly in fruit will be destroyed if the fruit is subjected to the fol- 
lowing treatment: 
" Cooling until the approximate center of the fruit in the package reaches 
a temperature of 30°-31° F. and holding the fruit at that temperature for 
15 days." 
Storage tests with some varieties of vinifera grapes, grown in the United 
States, have shown that the treatment can be applied to this fruit without 
danger of injuring it provided the requirements of the treatment as to tempera- 
ture are carefully followed. 
On the basis of the evidence secured provision is made for the entry, under 
permit and sterilization, of grapes of the vinifera type from regions in which 
the Mediterranean fruit fly occurs, at the port of New York and such other 
northern ports as may be subsequently approved, under the following con- 
ditions: 
(1) The grapes must be packed in tight barrels or kegs or other approved con- 
tainers so constructed as to prevent the escape from the container pending 
sterilization of any stages of the Mediterranean fruit fly, should they be present. 
Any broken containers wherever found must be immediately repacked under 
the supervision of an inspector of the Bureau of Plant Quarantine or the con- 
tents shall be immediately destroyed in a manner satisfactory to the inspector. 
(2) Within 24 hours from the time of unlading, the grapes shall be delivered 
for treatment to an approved sterilization plant. 
To provide necessary safeguards for movement to and handling at approved 
sterilization plants, those concerns designated to sterilize fruit are required 
to fde an application and complete a written agreement with the Bureau of 
Plant Quarantine. The Bureau will approve only those plants which are ade- 
quately equipped to handle and sterilize the fruit. 
