40 BUREAU OP ENTOMOLOGY AND PLANT QUARANTINE [April-June 
(2) The dosage for the fumigation shall be 3 pounds of liquid hydrocyanic 
acid or its equivalent per 1,000 cubic feet of space. 
(3) The air pressure in the fumigation chamber shall be reduced to the 
equivalent of 2 inches of mercury (a 28-inch vacuum at sea level), after which 
the hydrocyanic acid shall be introduced and the low pressure held for the 
duration of the fumigation. 
(4) The exposure shall be not less than 3 hours. 
2. Steam sterilization: 
(1) The air pressure in the treating chamber shall be reduced to the equivalent 
of 5 inches of mercury (a 25-inch vacuum at sea level). 
(2) Steam shall then be introduced until a positive pressure of 10 pounds is 
obtained. 
(3) The exposure to the 10-pound positive pressure of steam shall continue 
for a period sufficient to assure a constant temperature in all parts of the treat- 
ing chamber, after which the steam may be shut off and the treating chamber 
exhausted of the uncondensed steam. 
Avery S. Hoyt, 
Acting Chief, Bureau of Entomology and Plant Quarantine. 
ANNOUNCEMENTS RELATING TO JAPANESE BEETLE QUARANTINE 
(NO. 48) 
REVISION OF REGULATIONS 
INTRODUCTORY NOTE 
An important change in the following revision of the regulations of the Japa- 
nese beetle quarantine is the inclusion in the regulated areas for the first time 
of part of Schuyler County, N. Y., parts of the Ohio counties of Coshocton, 
Portage, and Summit, and parts of the West Virginia counties of Berkeley and 
Jefferson. This action is considered necessary because of the establishment of 
the Japanese beetle therein. Areas were also added to counties, parts of which 
were formerly under regulation, in the States of New York, Pennsylvania, Ohio, 
Maryland, and Virginia. Lancaster County, Pa., has been added to the special 
area (regulation 5, sec. A (1) (ii)) from which the movement of fruits and 
vegetables by motortruck or refrigerator car is regulated. Wheeling, W. Va., 
and Coshocton, Ohio, are brought under regulation and placed in the same cate- 
gory as Buffalo, Cleveland, and other outlying cities in that a certificate or 
permit is required in the movement of fruits and vegetables to these cities but 
no restrictions are placed on the interstate movement therefrom. 
Of interest to nurserymen is the elimination of restrictions on the movement 
of aquatics except during the period from June 15 to October 15, inclusive. 
The restrictions on the movement of sand are modified to exempt from certifi- 
cation silica sand, greensand, marl, "bird sand," "bird gravel," and pottery clay, 
when free from vegetable matter, and when labeled as to contents on the outside 
of each container. 
Some outlying areas where Japanese beetle infestations have been found the 
past field season are not included in the regulated area because of assurance 
from the States concerned that adequate measures will be taken to prevent the 
spread of the pest therefrom. 
summary 
Unless a certificate or permit has been issued, these regulations as now 
revised prohibit the interstate shipment of green corn on the cob, beans in the 
pod, bananas in entire bunches or in clusters of 25 or more, apples, peaches, 
blackberries, blueberries, huckleberries, or raspberries from the regulated areas, 
to or through points outside; and also prohibit (unless a certificate or permit 
has been issued) the interstate movement of all fruits and vegetables by re- 
frigerator car or motortruck from the District of Columbia, the State of 
Delaware, and parts of the States of Maryland, New Jersey, Pennsylvania, and 
Virginia, to or through points outside the regulated areas as defined in regula- 
tion 3. Refrigerator cars used for loading fruits and vegetables, other than 
onions and potatoes, in such area must, prior to loading, be cleaned by the 
common carrier and kept tightly closed and sealed during the interval between 
