- U - 
dorffi, longiflora , paniculata , p lumba gi ni fo li e, t guadrivalvis , repand.a , 
rusbyi , rustics,, Sf.rier^e, suaveolens , sylvestris , taba.cum. The alkaloid 
content of a number of hybrids is given. — ■ Shmuk and Xhmura (68). 
Ni c o t i ana t aba cum normally contains nicotine but no anabasine, whereas 
N. glauca contains only anabasine. When N. glauca w as grafted on N.tabacum, 
formation of the characteristic alkaloid proceeded in stock and scion with 
a consideraoxe increase in the anaba.sine content of th« scion over that of 
the control plant. Anabasine appeared to be formed in the stock also. When 
N. tabacu m was grafted on N, glauca , nicotine was completely replaced by 
anabasine in the scion while a trace of nicotine appeared in the stock. — 
Shmuk, Kostov, and Borozdina (69) . 
When Ni cotiana rustica was grafted to N. glauca , it lost nicotine and 
accumulated anaofsine. — Shmuk (63) . 
The grafting of nicotine-containing tobacco on the nicotine-free species, 
Nicotiana glauca , resulted in plants in which the nicotine was completely 
replaced by anabasine. — Evtushenko ( 22) . 
A study was made of the inheritance of nicotine and anabasine content 
by Nicotiana tabacum x Nicotiana glauca hybrids, and interaction of stock 
and scion when these species are grafted. In hyurids a change was noted 
in the type of alkaloids with reference to position of the leaves. — 
Kuz'menko and Tikhvinskaya (35) » 
Nornicotine was identified in the following species of Nicotiana : 
bentha m ian a, ingulba , longiflora , palmeri , sanguin ea, rolanifolia, 
suaveolens , sylvestris , and trigonophylla . — ShinukTo^) . 
A large number of representative Nicotiana species and hybrids were 
examined, for alkaloids. Ail 29 of the wild species contained 1 or more 
alkaloids. N. alata , bigelovii , go ssei , and wigandioides apparently con- 
tained only nicotine, N. glutino°.q t iraritima , o_top_hora, tomentosa , and 
trigonophylla apparently contained only nornicotine. N, debney i and g lauca 
contained largely d,l_- anabasine. In debneyi about 15 percent of the total 
alkaloid was nicotine, whereas in glauca a much lower percentage of this 
alkaloid was found. The remaining 18 species contained mixtures of nicotine 
and nornicotine. Nicotine was predominant only in N. benavidesii , langsdorffi , 
and stocktoni ; in longiflora and plumb agini folia it was not possible to de- 
termine which of the two alkaloids '> r as present in the higher concentration; 
and in the rest nornicotine predominate J.. No wild species studied had as 
much as 2 percent total alkaloid content, and. most species Had less than 0.5 
percent. In 23 species 8U to 100 percent of the total alkaloid content was 
taken up by the main alkaloid. In crosses between N. tabacum, which contains 
mostly nicotine, and species whose alkaloid complex was made up largely or 
entirely of nornicotine, the hyorids contained mainly nornicotine, together 
with small amounts of nicotine. The F]_ and amphidiploid N« tabacum (nicotine) 
x N. glauca (anabasine) contained mostly anabasine with some nicotine. In 
one sample of this 7\ a trace of nornicotine was detected. — H. H, Smith 
and C R. Smith ( ]k) . 
A method is presented for classifying tobacco^ broadly into three 
types according to their picrate melting points — (l) the 
