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A rapid method is proposed for determining total alkaloids 
in semimanufactured produots and waste materials in the production 
of anabasine* The alkaline extract is extracted with ethylene 
di chloride and the ethylene di chloride extract titrated with 
hydrochloric acid (litmus indicator)* For determination in 
Anabasis the raw material is extracted with water, a part of the water 
extract is made alkaline, and the analysis conducted as above*— 
SokoloT (7fi)* 
Separation of anabasine from nicotine and other alkaloids 
A titration method for determining the total alkaloid content 
of a mixture is proposed which is based on the fact that the acetyl 
derivative of anabasine does not form a pi orate and niootine does*— 
Khmura (53)* 
Anabasine can be separated from the alkaloids associated with 
it in Anabasis aphyll a by precipitation as f luosilicate from a solu- 
tion in ethyl or methyl alcohol* The fluosilicate, CjoHi4N2H2SiF6 • 
H2O, m*p* 239°(dec*), can be converted to the free base by reaction 
with sodium hydroxide* Anabasine is precipitated with alcoholic 
fluosilicio acid from an alcoholic medium* The precipitate is 
dissolved in hot water and titrated with a 0*1 N sodium hydroxide 
solution (thymolphthalein indicator)* Nicotine can be determined 
similarly* Nicotine can be distinguished from anabasine by the 
solubility of its fluosilicate in methyl aleoholj fluosilicates 
of both are insoluble in ethyl alcohol*— Sokolov ( 75 , 77)* 
A comparative study was made of the reactions of anabasine, nioo» 
tine, and oaniine* The Meltser reagent (carbon disulfide, ethyl 
alcohol, and dilute copper sulfate solution) causes a white turbidity 
with nicotine and a black-brown turbidity with anabasine and ooniine* 
All three alkaloids form characteristic crystals In saturated aqueous 
solution of piorio acid* An ether solution of anabasine or niootine, 
when mixed with an ether solution of iodine, first becomes turbid 
and then crystallises* Coniine does not undergo this reaction* 
Hecke reagent (selenic acid and concentrated sulfurio acid) colors 
the anabasine residue pink, but does not change the oolor of nico- 
tine or ooniine with hydrochloric aoid (sp* gr* 1*19)* The coniine 
residue forms beautiful violet crystals after evaporation* No 
such orystals are formed from anabasine or nicotine* With nltrio 
aoid (sp* gr* 1*4) the niootine residue assumes an Slow heating a 
pink oolor, which changes to green* Under the same conditions no 
coloration of anabasine and ooniine is observed* A maximum yield 
up to 95 percent of anabasine alkaloids is obtained by extraction 
of cadaver material with acidified alcohol*— Ratvadovskii (65). 
The following directions for determining niootine in the presence 
of anabasine are givent Treat the aqueous solution of the alkaloids 
with 3 ml* of 10-percent sulfurio aoid and 10 ml* of freshly pre* 
pared 5-peroent sodium nitrite, heat for 30 minutes at 40-60* # 
neutralise approximately by introducing 10-percent sodium hydroxide 
and then aoourately by titration with 0*1 N sulfurio aoid (with 
methyl orange), and add 3 to 4 drops of exoess aoid* Precipitate 
the picrates by addition of piorio aoid (12 gm* per liter) and cool 
for 4 hours* Filter, wash twice with 0.1-peroent piorio aoid and 
twice with water, transfer to a glass-stoppered flask, add 16 to 20 
