-u- 
In general, the method used is the same as for the fuchsin dye 
except that a 10-mlnute period is required to obtain maximum staining. 
An iodine solution should always be fresh -when used because it quickly 
loses its efficiency upon standing. It is also important to examine the 
grain soon after staining because the color gradually decreases in 
intensity when exposed to the air. 
Figure 1. — Wheat kernels showing egg-plugs of the granary weevil stained 
with acid fuchsin. 
