- 37 - 
This gives the weight :f pure rotonone contained in the .aliquot of the 
extract, representing 20 grans of the sample." 
Reference to this method of Jones and Grahan for the determination of 
rotcnonc in cube and derris is made .in the News Letter (227) of the Bureau of 
Entomology and Plant Quarantine. 
USE OF LOHDHOC.JffUS AS AI\ T INSECTICIDE 
ProhahlT" the earliest published account of the use of cube as an in- 
secticide occurs in an article by Klinge (245) in the February 1910 Bolctin 
de la Direccion de Foment o, Lina, Peru. A translation from the Spanish is as 
follows: 
"At present I an studying and testing out a liquid 1 for the con- 
trol of ticks on lianas of uhich I spnko in one of my recent letters. 
There is a plant here called "CUBS" in the Qucchuan dialect which is 
used for catching fish in darned up streans by poisoning the water with 
the root. Fron tests that I a/c it results that maceration produces 
an effective insecticide, which destroys the tick; but I dc not know 
whether it will be toxic to the lianas, if these aninals should take it 
accidentally internally, as would be the case in a dipping process. I 
an naking solutions '"oy crushing and naceration in various proportions, 
in order to find one, if possible, which kills the tick anr 1 is not 
poisonous to the lianas in quantities larger than one liter, which is 
the maximum that can be taken during a dipping bath. The work is done 
in the laboratory of the collc f e anr" 1 in a peasant house in the neighbor- 
hood of the town. This will delay nc a few no re days." 
Dclassus, Lepigre, and Pasquier (112) in 1933 wrote that rotonone (fror. 
the roots of Derris, Tephrosia, Lonchocarxms , etc.) nay be used like pyre thru" 
insecticides against insects attacking grapevines in Algeria. 
The British Guiana Department of Agriculture (48) in 1934 published 
results of tests of cold water, hot water, and carbon tetrachloride extracts 
of the bark, stens, and loaves of fresh young shoots of haiari against the 
red stinging ant, Solon^psis sp. Hone of the extracts had any effect on the 
ants. An aqueous extract of haiari gave . excellent results against another 
species of ant, probably Prcnolcpis sp. , infesting coffee trees. 
Garman and Turner (158) in March 1934 published information on sub- 
stitutes for lead arsenate on fruits and vegetables in Connecticut. Rotonone 
preparations node fron cube or derris are promising both as stomach and con- 
tact poisons, and are recommended against insects attacking currants, goose- 
berries, raspberries, strawberries, beans, cabbage, cauliflower, broccoli, 
celery, and leafy vegetables such as beets, turnips, lettuce, spinach, Swiss 
chard., New Zealand spinach, etc. 
The United States Department "f Agriculture, Bureau of Sntonology (417), 
on March 14, 1934, '.issued a mimeographed memoranda- recommending derris or 
