-u- 
A drone is anesthetised by droppingMm in the vial already described. 
His abdomen contracts and usually the penis partially everts, as illus- 
trated in figure 10, A, He is then made to evert more completely by 
being squeezed between thumb and forefinger. There is great variation 
in the degree of ever s ion, the distribution of semen and mucus, and the 
amount of semen ejaculated* Bversion usually stops at the stage illus- 
trated in figure 10, B^, which is about two-thirds complete. As the pro- 
cess of eversion proceeds, the cream-colored semen passes out first fol- 
lowed by the thicker white mucus* If the eversion process can be stopped 
at just the right point, a drop of pure semen will be found at the tip 
with all the mucus left inside. IVhen this ideal situation exists it is 
very easy to take up the semen. Usually, however, at least some of the 
mucus comes out after the semen, and the two are distributed on the penis 
in various arrangements. Often the semen is spread so thinly over the 
mucus that it is difficult to take up. 
It is best to see that an ample supply of drones is available and to 
use only those that evert and ejaculate most satisfactorily. This is 
especially important when individual matings are being made, so that the 
maximiam amount of semen is obtained from the single drone used. Movement 
of sperm causes the semen to spread in a thin layer over the mucus and to 
mix with it until too thick to be taken into the syringe easily. This 
mixing also takes place while the drone is partially everted, ^t is, 
therefore, important that drones be utilized as soon as possible after 
anesthetization. Without abdominal contraction semen is rarely obtainable, 
but when the abdomen contracts without partial eversion the eversion can 
often be completed by pressure, and a good amount of semen obtained. 
The ejaculated drone is brought near the tip of the syringe with the 
left hand and the plunger withdrawn slightly to make an air bubble. The 
surface of the semen is then made to touch the point of the syringe at 
about a 45-degree angle. If the syringe is raised slightly after contact 
has been made, the semen will adhere to it and flow toward it as the plung- 
er is withdrawn. This procedure helps to avoid the mucus, which is more 
viscous and will not flow so easily as semen.. Mucus is too thick to pass 
through the end of the syringe easily and will stop the passage of semen. 
"kVhen this happens, the plunger is pushed out until the passage is clear- 
ed and then the. taking of semen is continued. By moving the syringe 
about, the mucus 6an be skimmed of practically all its semen covering. 
Semen is taken from as many drones as necessary to fill the syringe to 
the desired point. 
As the syringe is filled, it will be noticed that the suction pulls 
some of the water from around the plunger, thus increasing the amount 
above the air bubble. Later, as the plunger is pushed out during injec- 
tion, this water passes back around the plunger. This action will be 
negligible with a well-made tip. When the plvinger is poorly fitted, 
however, it is sometimes withdrawn as far as it will go before the 
desired amount of semen has been drawn up. In this case the plunger can 
be pushed down again while a finger is being held over the end. Such a 
tip is a time consumer and should be replaced as soon as possible. Some 
water should remain between the end of the plunger and the air bubble to 
pass back around the plunger as the semen is injected; otherwise the air 
bubble, or oven some of the semen, may pass around the plunger. 
