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trlangular area between the basal portions of the sting lancets, and 
extends vmderneath them (fig. l) • Figure 9 gives' a side view of this 
Instrument at A and a top view at B^ from the angle Indicated by the arrow 
In A. The enlarged tip Is 0»77 mm« wide. The thinnest part of the stea 
is 0*08 mm« when viewed from above and 0*17 mm. when viewed from the side* 
The stem is bent to fit the queen parts. This Instru&ent is made from 
wire about 0«92 mm. in diameter, and filed down to the proper shape with 
jewelers' files. The enlarged tip represents the end of the wire filed 
off at an angle. 
All rough edges of both the ventral and sting hook are polished first 
with a fine stone and then with Jewelers* rouge. Both hooks are soldered 
to the ends of brass rods 3/l6 inch in diameter and 5 inohes long, irtiloh 
serve as handles and fit into the sheet-metal boxes* 
Other Equipment 
Other items of equipment needed for the operation are a pair of for- 
ceps, a sting depressor, a dish of water for cleaning the syringe, a vagi- 
nal probe, and a container for treating drones with chloroform* 
The sting depressor is simply a dull-pointed dissecting needle. 
The probe (fig. 9) is an instrument used to push the valvefold down- 
ward. It is a piece of No* 24 gage brass wire bent at rigjit angles 4 mm* 
from one end* This end is flattened to a thickness of 0*1S mm* on a plane 
with the main stem of the instrument and polished down to a width of about 
0*19 mm. The probe is bent slightly near the tip, so that the main part 
of the wire does not obstruct the view when the tip is Inserted into the 
vagina* 
Drones are made to ejaculate in a l-by-4-inch glass vial containing 
paper soaked in chloroform wadded in the bottom* A stopper fastened to 
the table so that the vial can be put onto it in a horizontal position 
is a great time saver* 
INSEMINATION PROCEDURE 
Adjusting the Flow of Carbon Dioxide 
The end of the carbon dioxide supply tube is iamersed in water and 
the flow of gas adjusted to a slow bubbling. It should not be faster than 
.is necessary to keep the queen completely anesthetlred. Experience will 
soon show just how much this should be. The tube is then passed through 
the queenholder mounting block from below, the stopper attached, and the 
three-way stopcock in the supply line turned, to prevent gas from passing 
through the stopper while the queen is being mounted. 
Preparing the Syringe 
To function properly the tip of the glass syringe must be filled 
with water, which acts as a lubricant and increases the suction of the 
plunger. Vlater is firdt drawn into the tip by withdrawing the plunger* 
