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CONTENTS 
Page 
Introduction • • • 3 
The reproductive organs ..... 3 
Bquipnent and oonstruotion of instruments ...•••..... 5 
Miorosoope and light •• ....... 5 
Carbon dioxide equipment ••••« •• 5 
The manipulating appeu-atus 6 
Queen holder 6 
Syringe •• 7 
Glass syringe .* • 8 
Plastic syringe • 10 
Holding hooks «.•••• . . 11 
Other equipment • 12 
Insemination procedure • 12 
Adjusting the flow of carbon dioxide •........• 12 
Preparing the syringe •..••• • 12 
Preparing the queen 13 
Filling the syringe IS 
Injection 15 
Cleaning the syringe • 15 
Sterilizing the syringe • • 16 
Estimating the number of sperms 16 
The use of carbon dioxide 17 
Procedures for Tarious types of matings and results to be 
expected •• • 18 
Individual matings • 18 
One insemination with sperm from several drones 19 
Two or more inseminations 19 
The absolute control of parentage 20 
Care of queens ••• • 21 
Bearing and care of drones 22 
Summary • •••.••• 24 
Literature cited 26 
