1942] 
SERVICE AND REGULATORY ANNOUNCEMENTS 
31 
B. E. P. Q. 499 Effective May 7, 1942 
Supplement No. 4, First Revision 
TITLE 7— AGRICULTURE 
Chapter III — Bureau of Entomology and Plant Quarantine 
Part 301 — Domestic Quarantine Notices 
japanese beetle administrative instructions modified 
Pursuant to the authority conferred upon the Chief of the Bureau of Entomology 
and Plant Quarantine by §301.48-6, Chapter III, Title 7, Code of Federal Regu- 
lations [regulation 6 of the rules and regulations supplemental to Notice of Qua- 
rantine No. 48 on account of the Japanese beetle], subsections (i) (4), (k) (1), 
and (m) (2) of § 301.48b [see pages 6, 8, and 15, respectively, of the mimeographed 
edition of circular B. E. P. Q. 499. issued June 9, 1939], are hereby modified, 
effective May 7, 1942, to read as follows: 
§ 301.48b Administrative instructions to inspectors on the treatment of nursery 
products, fruits, vegetables, and soil, for the Japanese beetle 
(i) POTTING SOIL 
(4) Lead arsenate treatment 
Season. — The treatment must be applied before August 1. 
Condition and type of soil.— The soil must be friable. Wet soil must never be 
treated. The treatment is recommended only for soils that are slightly acid or 
neutral in reaction. Any type of soil may be treated provided it meets these 
requirements. 
Dosage. — Two pounds to 1 cubic yard. 
Application. — The lead arsenate must be thoroughly mixed with the soil. 
Period of treatment. — Plants freed from soil and potted in soil treated in the above 
manner, by August 1, may be certified for shipment between the following October 
1 and June 15, inclusive. 
Handling of potted plants. — When plants potted in lead-arsenate-treated soil 
are plunged in beds or set in frames exposed to possible infestation, the soil of these 
beds or frames must previously have been treated with lead arsenate at the rate 
of 1,000 pounds per acre. 
Treated plants carried after June 15. — When plants potted in soil treated as 
prescribed are carried after June 15, they may be again eligible for certification 
between October 1 and June 15, inclusive, of the second year if, on August 1 of 
the second year, analyses show the soil to contain lead arsenate at the rate of 2 
pounds per cubic yard. 
(k) SOIL IN AND AROUND COLDFRAMES, PLUNGING BEDS, AND HEELING-IN AREAS 
(1) Lead arsenate treatment 
Season. — The treatment must be applied before August 1 if the land is to be 
used in the fall. 
Condition of soil. — The soil must be friable and in good tilth. 
Dosage. — Twenty-three pounds to each 1,000 square feet, or 1,000 pounds per 
acre. For subsequent re-treatments, the quantity required to restore a concen- 
tration of 1,000 pounds per acre, as determined by chemical analyses, must be 
applied, except that determination by chemical anatyses of a concentration of 
900 pounds per acre will be acceptable without re-treatment. 
Application.— The lead arsenate must be thoroughly mixed and incorporated 
with the upper 3 hrehes of soil. 
Period of treatment. — Plants must not be placed on or in the soil thus treated 
until after October 1. 
(M) TREATMENT OF PLANTS BEFORE DIGGING 
{2) Lead arsenate treatment 
Season. — Treatment must be applied by July 1. Plants may be certified when 
the period of treatment is completed, and until the following June 15. 
Condition of soil. — The soil must be friable and in good tilth. This treatment 
is recommended only for soils that are slightly acid or neutral in reaction. 
