32 BUREAU OF ENTOMOLOGY AND PLANT QUARANTINE [April-June 
Dosage. — Twenty-three pounds to each 1,000 square feet, or 1,000 pounds per 
acre. For subsequent re-treatments, the quantity required to restore a concen- 
tration of 1,000 pounds per acre, as determined by chemical analyses, must be 
applied, except that determination by chemical analyses of a concentration of 
900 pounds per acre will be acceptable without re-treatment. 
Period of treatment. — Plants in plots trexted initially must not be dug until 
October 1; those on re-treated plots may be dug on September 20. 
Application. — Lead arsenate must be thoroughly mixed and incorporated with 
the upper 3 inches of soil. The ridge of soil between the plants in the rows and 
the soil about the base of the plants must be removed to a depth of 2 inches and 
placed in the space between the rows of plants. Lead arsenate may be applied 
with a suitable distributor or broadcast by hand, before or after the hoeing 
operation is completed. Then the soil between the rows of plants must be cul- 
tivated three times. On the last cultivation, the cultivator is adjusted in such 
a manner that the treated soil is thrown toward the rows of plants. At least 
3 inches of treated soil must be placed in the rows about the bases of the plants. 
Varieties of plants. — The varieties of plants which have been treated successfully 
by this method are given in Bureau of Entomology and Plant Quarantine Circular 
E-418. 
Safety zone. — Same as that prescribed in (k). 
Marking. — Same as that prescribed in (k). 
(7 C. F. R. § 301.48; sec. 8, 39 Stat. 1165, 44 Stat. 250; 7 U. S. C. 161.) 
Done at Washington, D. C, this 2d day of May 1942. 
P. N. Annand, 
Chief. 
B. E. P. Q. 499, Effective May 18, 1942 
Supplement No. 5 
TITLE 7— AGRICULTURE 
Chapter III — Bureau of Entomology and Plant Quarantine 
Part 301 — Domestic Quarantine Notices 
japanese beetle administrative instructions modified 
INTRODUCTORY NOTE 
Experience and further experiments in paradichlorobenzene fumigation for the 
treatment of plants after digging to free them from infestation by Japanese beetle 
permit modification of treating requirement approved June 9, 1939, without 
increasing risk of spread. The instructions authorizing the use of this method are 
accordingly revised to reduce the period of treatment from 5 to 3 days. 
Pursuant to the authority conferred upon the Chief of the Bureau of Entomologv 
and Plant Quarantine by § 301.48-6, Chapter III, Title 7, Code of Federal Regu- 
lations [regulation 6 of the rules and regulations supplemental to Notice of Quar- 
antine No. 48 on account of the Japanese beetle], paragraph (1) (4) of § 301.48b 
[see page 11 of the mimeographed edition of circular B. E. P. Q. 499, issued June 9, 
1939] is hereby modified effective May 18, 1942, to read as follows: 
§ 301.48b Administrative instructions to inspectors on the treatment of nursery 
products, fruits, vegetables, and soil, for the Japanese beetle. * * * 
Teeatment of Soil About the Roots of Plants 
(l) treatment of plants after digging * * * 
(4) Paradichlorobenzene fumigation 
Season. — The treatment must be applied between October 1 and May 1. 
Varieties of plants. — Many different kinds of plants have been successfully 
treated experimentally. The list of plants which have been treated without 
injury is subject to such continual expansion that it cannot be appropriately 
included in these instructions. Experience has shown that possible plant injury 
is associated at least to some extent with the condition and growth of the plants 
at time of treatment. It is suggested, therefore, that trial tests be made before 
large numbers of plants are treated. 
