54 BUREAU OF ENTOMOLOGY AND PLANT QUARANTINE [July-Sept. 
singly or in groups with the halls spaced approximately % inch apart in a wire 
basket or perforated tray, and arranged so as to permit of rapid penetration 
of the emulsion into all of the balls. In any case the plant balls or tray shall 
rest on the bottom of the tank. A sufficient quantity of freshly prepared, 
diluted emulsion shall be added to the dip so that the plant balls are completely 
covered during the immersion period. To reduce the hazard of plant injury, 
not more than the lower y 2 inch of the plant steins should be immersed during 
the treatment. The contents of the trough shall be discarded and the trough 
rinsed out 4 hours after charging and/or when the dirt and debris exceed 2 
inches in depth. The trough shall be located during plant treatments in a 
covered and well ventilated place. On removal of balled plants from the dip 
they may be allowed to drain into the tank for 1 or 2 minutes and then must 
be placed in a compact group either on a bench with a tight bottom and side 
walls as high as the plant balls, or on a tight floor of a greenhouse, packing 
shed or other enclosed area, and surrounded by wodoen side walls as high as the 
plant balls. If they are placed on a dirt floor it must be wet and packed hard 
before using. In the case of potted plants any excess emulsion should be 
poured from the pot immediately after removing from the dipping vat. All 
plants must remain undisturbed for the prescribed 48 hours during which time 
excessive ventilation should be avoided. A light spray of water applied to the 
tops of the plants during this period may be beneficial. 
(x) Period of treatment. — Ten seconds immersion in the dip followed by a 
48-hour holding period. 
(xi) Varieties of plants. — The list of plants which have been successfully 
treated in experimental work includes 18 varieties of azaleas, 60 kinds of 
greenhouse plants, 48 kinds of perennials, and 28 kinds of trees and shrubs. 
The list is subject to expansion and will be furnished on requet. 
(7 C.F.R., § 301.48; sec. 8, 39 Stat. 1165, 44 Stat. 250; 7 U.S.C. 161.) 
Done at Washington this 4th day of September 1942. 
P. N. Annand, 
Chief. 
[Filed with the Division of the Federal Register September 9, 1942, 11 : 08 a. m. ; 7. F. R. 
7134.] 
B. E. P. Q. 499, Effective September 18, 1942. 
Supplement No. 1, Sixth Revision. 
TITLE 7— AGRICULTURE 
Chapter III — Bureau of Entomology and Plant Quarantine 
Part 301 — Domestic Quarantine Notices 
japanese beetle administrative instructions modified 
INTRODUCTORY NOTE 
Two new schedules for methyl bromide fumigation of potted or bare-rooted 
plants are provided in this revision of supplement No. 1. These two treating 
schedules, at lower temperatures than have heretofore been authorized, are Nos. 
8 and 9 in the table under subparagraph (i). The instructions as to fumigation 
of packaged plants are carried forward in this revision of the supplement. 
§ 301.48b. Administrative instructions to inspectors on the treatment of 
nursery products, fruits, vegetables, and soil, for the Japanese beetle. Treat- 
ment authorized. Pursuant to the authority conferred upon the Chief of the 
Bureau of Entomology and Plant Quarantine by § 301.48-6, Chapter III, Title 7, 
Code of Federal Regulations [regulation 6 of the rules and regulations supple- 
mental to Notice of Quarantine No. 48], subsection (1) (5) of § 301.48b [on 
page 13 of the mimeographed edition of circular P». E. P. Q. 499. issued June 9, 
1939] is hereby further modified effective September 18, 1942, to read as follows: 
