YttAHSU 
WAOtt TVuUS 3TATB 
56 BUREAU OF ENTOMOLOGY AND PLANT QUARANTINE [July-Sept. 
B. E. P. Q. 499, Supplement No. 7. Effective September 18, 1942. 
TITLE 7— AGRICULTURE 
Chapter III — Bureau of Entomology and Plant Quarantine 
Part 301 — Domestic Quarantine Notices 
japanese beetle administrative instructions modified 
INTRODUCTORY NOTE 
Experiments with methyl bromide dissolved in water and applied to specified 
soil areas have resulted in the development of new methods for treating the 
soil of areas free from plants and of individual items of nursery stock in field 
rows. The application of this treatment in meeting the requirements of the 
Japanese beetle quarantine must be conducted under the supervision of an 
inspector of the Division of Japanese Beetle Control, 266 Glenwood Avenue. 
Bloomfield, N. J., and in accordance with detailed instructions furnished by 
him. 
Pursuant to the authority conferred upon the Chief of the Bureau of Ento- 
mology and Plant Quarantine by §§ 301.48-6 and 301.48-7, Chapter III, Title 7, 
Code of Federal Regulations [regulations 6 and 7 of the rules and regulations 
supplemental to Notice of Quarantine No. 48], paragraphs (k) and (m) of 
§ 301.48b [circular B. E. P. Q. 499, issued June 9, 1939], as amended, are hereby 
further amended effective September 18, 1942, by the addition of the following 
subparagraphs : 
§ 301.48b Administrative instructions to inspectors on the treatment of nursery 
products, fruits, vegetables, and soil, for the Japanese beetle. 
******•„ * • 
Treatment of Soil in Absence of Plants 
******* 
(k) SOIL in and around coldframes, plunging beds, and eteeling-in areas 
* * * 
(6) Methyl bromide solution 
(i) Season. — The treatment can be applied at any time when conditions are 
suitable between October 1 and May 15. 
(ii) Equipment. — Equipment includes a gastight drum, complete with spigot 
and hose, methyl bromide applicator, collars when necessary, and measuring cans. 
Such equipment must be inspected, tested, and approved by an inspector of the 
Department before use. 
(iii) Preparation of solution. — The solution must be prepared in accordance 
with the directions of the inspector. 
(iv) Condition and type of soil. — Soil of any type may be treated provided the 
surface can be pulverized sufficiently to absorb the solution. To prepare a well 
pulverized surface, areas to be treated must be leveled and thereafter cultivated 
to loosen the soil to a depth of at least 1 inch. The treatment must not be applied 
during rain. The surface of wet soil should be tilled, allowed to dry for at least 
24 hours, and then pulverized preparatory to treatment. 
(v) Dosage and application. — The dosage shall be at the rate of 3 gallons of 
solution per 1 square yard. The strength of the solution shall be based on the 
minimum soil temperature within the top 6 inches as follows: 
Minimum soil temperature in Percentage concentration by volume of 
top 6 inches (°F. ) : methyl bromide 
47 to 56, inclusive 0. 150 
57 to 67, inclusive . 100 
68 and over . 050 
The surface must be divided by strings or marks in the soil into units of approxi- 
mately 1 square yard. The solution is to be applied uniformly in a crisscross pat- 
