-14- 
animals were placed in a hermetically sealed glass chamber of 409.7 
liters capacity, a 1-pound aerosol cylinder was discharged into the 
closed chamber, and the animals were exposed to the resulting mist 
for 46 minutes daily, 6 days a week, for 1 montii. Under these con- 
ditions APS-202 is not a primary skin irritant and, unlike Vel6icol 
KH70, it is not photosensitive when applied to the skin. Aerosol No, 
305 is no more toxic than No, 2730 (contains 3 percent of DDT), 
studied previously, and its use as an insecticide should not involve 
any hazards.— von Oettingen et al, (363), 
In solid form DDT applied topically to the skin is nonirritating, 
nonsensitizing, and not appreciably absorbed. In solution, either in oil 
or in organio solvent, it readily penetrates the skia and is mildly irri- 
tating and sensitizing. In single and multiple dose administration (acute 
and subacute) there are wide individual as well as wide speoies variations. 
In prolonged feeding experiments (chronic toxicity) rats have been fed 
diets containing 100, 200, 400, 800 ppn. of DDT for about 18 months. 
Guinea pigs, dogs, and monkeys have been studied for shorter periods* 
The pharmacological manifestations of effect from DDT are principally loss 
of appetite, mild to severe tremors of central nervous system origin, 
convulsions, and death. Tremors can be prevented or abolished by general 
anesthetics and narcotics* Histopathologic examination of tissues of 
animals which have received DDT shows damage which is neither striking 
nor characteristic for all speoies,— Calvery (103), 
DDT is cumulative in effect. "Whether this central nervous system 
effect is reversible we do not know. The tremors can be completely 
abolished by sedatives, and in my opinion if this is done that animal is 
recovered. But if it has gone on long enough to affect the internal or- 
gans, that may not be reversible. Except sedatives, there is no specific 
treatment for DDT poisoning ."—Calvery (104), 
Typical signs of 17DT poisoning «bserved on mammals under laboratory 
control were lessened appetite with corresponding weight loss, nervous- 
ness, tremors, and in the final stage convulsions. Tho liver and thyroid 
may be affected and, after repeated application to the skin, a slight 
dermatitis may occur. The exposure to large doses of undiluted DOT in 
powdered form produced no synptoms of poisoning in animals with either 
intact or abraded skin. However, solutions of DDT in a nonirritant sol- 
vent such as dimethyl phthalate caused severe poisoning. No irritation 
was noted from powdered DDT on the hands of operators who had almost 
daily contact with it during the past year, A number of commercial pre- 
parations containing up to 6 percent of DDT have proved safe for limited 
use. The inunction of doses as low as 0*5 ml, of a 30 peroent solution 
of BDT per kg, per day (150 mg, per kg. per day of DDT) to rabbits, rats, 
and guinea pigs may cause death in some cases after 30 days. Affected 
