554 
BEES AND BEE-KEEPING. 
carried by the needle, as before, into a second tube, the 
spores germinate, bacilli developing within, breaking 
the capsule, and escaping as seen at g, h, z, k, /, D : 
multiplication by fissuration now occurs about ever)' 
twenty minutes. If the tubes be inoculated in suc- 
cession, it is evident that everything drawn from the 
Fig. 122.— Bacillus alvei under Cultivation. 
A, Colonies of Bacillus alvei under Cultivation (6 diameters). B, Same Colonies 
Twenty-four Hours later. C, Culture Tube (Scale, \)—ffl, Gelatine; p, Plug. 
D, Spore becoming Bacillus (1800 diameters). E, Bacillus becoming Spore. 
F, Spores in Line from a Culture. G, Colony Spreading (50 diameters). 
bee whence the virus was taken will be quickly elimi- 
nated, the bacillus excepted. Twelve tubes were thus 
cultivated in series in the Biological Laboratory, Ken- 
sinorton, and then a culture was made in milk from the 
last. Some of this, containing countless bacilli, I 
sprayed over a comb of young larvae, part of which 
I screened by a cardboard having four large lozenges 
