80 
Philippine Journal of Science 
1920 
The trypanosome is a long, graceful organism, rather pointed 
anteriorly but usually having a bluntly rounded posterior ex- 
tremity. The stained preparations sho-w the protoplasm of the 
main portion of the body to be finely granular with a structure 
that is more or less alveolar except toward the posterior end, 
where the cytoplasm is clearer and more hyaline. In those 
individuals to which we refer, provisionally, as normal forms 
the round to slightly oval nucleus lies at about the middle of the 
body. Apparently the nucleus is of the protokaryon type with 
a rather small karyosome and abundant peripheral chromatin 
arranged around the internal surface of a nuclear membrane. 
The parabasal body " lies at about the beginning of the posterior 
fourth of the body. In some individuals we were able to dis- 
tinguish a finer granule, lying just anterior to the parabasal, 
which we are inclined to regard as the blepharoplast, for it 
seems to be associated with the origin of the flagellum. This 
is shown in Plate 1, figs. 8 and 9. Figs. 10 and 11 on the same 
plate also show two bodies, apparently in division. We are 
unable to determine if they represent the early division of a 
blepharoplast-centrosome, a division of the parabasal, or some 
wholly fortuitous element. Such interpretations are not to be 
made with any confidence in material that has undergone the 
treatment that this did. 
In individuals of the more normal type the undulating mem- 
brane was seen to be long and thrown into graceful folds. Un- 
fortunately no dividing forms were encountered. 
Of especial interest to us, however, was the relation between 
the nucleus and the parabasal shown in several individuals. In 
some cases this had brought about the development of what 
might be termed a pseudo-crithidial form of the parasite. There 
were instances where the parabasal lay anterior to the nucleus, 
the flagellum springing from its region and pursuing the usual 
anterior course as the margin of the undulating membrane and 
terminating anteriorly as a free lash. 
In view of the fact that cultural forms and those that have 
passed through subinoculation are not involved here, the condi- 
tion is somewhat novel— at least, it is novel to us in a country 
where Trypanosoma evansi is the only species of importance. 
‘We have adopted the term parabasal as employed by Kofoid and his 
coworkers in preference to Woodcock’s term “Kinetonucleus.” For a dis- 
cussion of the considerations here involved see Swezy, Olive, The kineto- 
nucleus of flagellates and the binuclear theory of Hartmann, Univ. Calif. 
Pub. in Zool. 16 (1916) 185. 
