Santos y Alvarez: Copra Meal 
183 
16, 2 
hour and then filtered. This treatment removed the water- 
soluble protein and a part of the salt-soluble, together with the 
starch and other soluble carbohydrates. The residue was washed 
thoroughly with hot water. The filtrate together -with the wash- 
ing was treated quantitatively for total nitrogen, and the residue 
hydrolyzed with 20 per cent hydrochloric acid (14) for forty-eight 
hours. The distribution of nitrogen in the hydrolysate was de- 
termined according to the directions in Plimmer’s monograph ( 19 ) 
with certain modifications, stated below. The residue from hy- 
drolysis was treated quantitatively for hydrochloric acid insoluble 
humin nitrogen in accordance with the method of Gortner and 
Holm. (6) 
Table 2 . — Distribution of nitrogen in copra meal {two-gram samples). 
Nitrogen 
in each 
sample. 
Nitrogen 
average. 
Different forms of 
hydrochloric-acid- 
Boluble nitrogen. 
Nitrogen 
in each 
sample. 
Nitrogen 
average. 
Total 
nitrogen 
insoluble 
in hot 
water. 
vig. 
mg. 
mg. 
mg. 
Per cent. 
Nitrogen in hot water 
1 14.1 
1 13. 35 
extract. ■> 
1 12.6 
Hydrochloric acid-in- 
1 1.41 
1 1.84 
1 1.41 
I 1.34 
2.24 
soluble humin nitro- 
1 1.27 
i 1.27 
gen. 
Humin nitrogen solu- 
1 
ble in hydrochloric 
acid. 
2.90 
1 15. 50 
1 15. 20 
2.90 
4.87 
Amide nitrogen 
1 15.35 
25. 72 
Arginine nitrogen 
1 7.00 
1 7.00 
1 7.00 
11.73 
Nitrogen soluble in 
hydrochloric acid. 
1 61. 14 
1 6B.62 
1 68.33 
Histidine nitrogen 
2.35 
2.35 
3.94 
Lysine nitrogen 
1.31 
1.31 
2. 19 
Cystine nitrogen 
f 4.05 
1 3.82 
1 3.93 
6.68 
Mono-amino nitrogen. 
I 25. 52 
1 24.57 
1 25. 04 
41. 96 
Non-amino nitrogen. 
1.24 
1. 24 
2.08 
Total nitrogen 
73.02 
60.46 
101.31 
“ The distribution of this nitrogen was not determined. 
Hydrochloric acid insoluble humin nitrogen (mg.) 1.34 
Hydrochloric acid soluble nitrogen (mg.) 58.33 
Total nitrogen insoluble in hot water (mg.) 59.67 
Nitrogen in hot water extract (mg.) 13.35 
Total nitrogen by direct analysis (mg.) 73.02 
Total nitrogen accounted for (per cent) 99.75 
