16,2 
Santos y Alvarez: Copra Meal 
185 
hydrochloric acid, thus removing starch and other soluble car- 
bohydrates, and the water- and part of the salt-soluble protein. 
The results are compared with the nitrogen distribution of lactal- 
bumin plus an equal weight of dextrose as reported by Gortner. (5) 
When compared with this protein (Table 3) copra meal is found 
to be richer in amide, humin, cystine, arginine, and histidine, 
and poorer in lysine, mono-amino, and non-amino-acid nitrogen. 
Compared with cottonseed meal, it is found to be richer in amide 
and cystine nitrogen, and poorer in arginine, lysine, and non- 
amino-acid nitrogen. The humin and mono-amino-acid nitrogen 
of both are practically the same. 
In short, copra meal, while rather poor in the sum total for 
lysine, (16) is rich in arginine, histidine, (l) and cystine. (17) The 
preliminary feeding experiments conducted by the animal hus- 
bandry department of the College of Agriculture seem to show 
that copra meal, when fed alone, cannot promote growth in hogs ; 
but, when fed with green leaves, it furnishes a fairly perfect 
feed. It is possible that copra meal is lacking in “unknown 
accessories” (10) which here appear to be supplied by the green 
leaves. 
The nitrogen partition in the hot-water-soluble proteins has 
not been studied. It is hoped that this will be done in the 
near future. Some idea of the distribution of nitrogen in these 
proteins may be gained by reference to the results with coconut 
globulin obtained by Osborne and Harris, and given in Plimmer’s 
monograph. (18) These results calculated to percentage of total 
nitrogen have been included in Table 3. 
DETERMINATION OF PROTEINS SOLUBLE IN DIFFERENT SOLVENTS 
The fat-free copra meal, dried at room temperature, was treated 
according to the method proposed by H. H. Snyder and reported 
by Harcourt.(9) The meal was treated Avith water and then 
with salt solution before extracting it with alcohol. Chloroform 
was added to prevent decomposition. In the first trial only 
about 30 per cent of the protein was extracted by the different 
solvents. This was thought to be due to the inability of the 
solvents to penetrate the particles of the copra meal, the sample 
used being a mixture of meals that had been passed through 
40-, 60-, 80-, and 100-mesh sieves, respectively. In the second 
trial only the sample passed through the 100-mesh sieve was 
used. It is regrettable that a finer sieve could not be obtained. 
The two samples gave the same amount of total nitrogen; so it 
trial only the sample passed through the 100-mesh sieve was 
was at first thought that they had the same composition. This 
