590 
Philippine Journal of Science 
1920 
deviations from this standard are negligible if only small quan- 
tities of glucose are involved in the analysis; but they become 
of great importance vv^hen relatively large amounts are present 
in the solution. 
Experiments show that the mercury thread begins to fluctuate 
approximately thirty seconds after the thermometer reaches 95°. 
The temperature at the end of the thirty seconds, and just when 
Milligrams of glucose. 
Fig. 5. Relation between milligrams of glucose and cubic centimeters of 0.06 N potassium 
permanganate solution reduced. Curves B are for 0.1 N and curves C, for 0.05 N potas- 
sium permanganate. Curve 1 is for 0.8 N alkali solution ; curve 2, for 0.6 N solution ; 
curve 3, for 0.4 N solution ; curve 4, for 0.2 N solution ; curve 6, for 0.04 N solution. 
fluctuation begins, has been observed to be about 104° to 
105° C. when the dilution is 100 cubic centimeters, and 110° C. 
when the dilution is 60 cubic centimeters. Boiling of the solu- 
tion starts within 5° before fluctuation begins. At this point, 
the rise of temperature is in most cases so rapid that it is impos- 
sible to time it accurately. 
THE INFLUENCE OP TIME AND TEMPERATURE UPON THE OXIDATION 
A series of experiments was performed to determine the 
influence of temperature and time of heating upon the degree 
