BUREAU OF ENTOMOLOGY AND PLANT QUARANTINE 91 
approximately the same growth rate during early life and then the 
growth of the worker caste is retarded, as shown by the amount of 
nitrogen, total lipid, total reducing substances, and calorific value. 
Queens attained a maximum live weight of more than 260 milligrams 
during development, as compared with 144 milligrams for the 
workers. Water comprised 76 to 85 percent of workers and 75 to 
83 percent of queens during development. The queens reached a 
maximum nitrogen content of 5.7 milligrams per individual, while 
the workers reached only 2.2 milligrams. The highest total lipid 
content was 12.8 milligrams for queens and 5.4 milligrams for 
workers. Total reducing substances reached a maximum of 13.2 
milligrams in queens and 12.1 milligrams in workers. The average 
ash content of the prepupal and pupal stages of the worker was 
approximately six times that of the queen. The calorific value of 
the tissues of both castes increased during the larval period and 
decreased during metamorphosis. 
EFFECT OF FEEDING ON WEIGHT OF QUEENS 
In a study of the effect of various factors upon the weight of 
the queen larva and the production of royal jelly, it was found that 
the weight of the larva was not correlated with the amount of 
food. It appears, therefore, that the prevailing effort among queen 
breeders to insure that the queen larvae be copiously supplied with 
larval food does not necessarily increase the weight of adult queens. 
Increasing the number of bees in a swarm box did not insure a 
proportionate increase in the amount of larval food, or an increase 
in the weight of the larvae. 
PROGRESS IN ARTIFICIAL INSEMINATION 
^ Considerable progress was made in the field of artificial insemina- 
tion. It has been shown that etherization may cause queens to lay 
more than one egg to the cell. Comparisons of spermathecae of 
queens inseminated by various methods showed that the greater 
the amount of sperm injected at a single operation the greater the 
degree of insemination and that some method of closing the opening 
of the vagina with mucus from the mucus gland of the drone is 
important. 
NOTES ON MATING 
The twenty-fifth floor of a building was found unsuitable as an iso- 
lated mating station when other bees were on the ground in the 
vicinity. 
Observations on natural matings show that approximately half of 
the queens of each of three strains mated twice, usually on successive 
days. 
INVESTIGATIONS OF INSECTS AFFECTING MAN AND ANIMALS 
SCREWWORMS AND BLOWFLIES 
Sufficient experimental data were obtained on the use of the new 
chemical diphenylamine in protecting wounds of animals from screw- 
worm infestations so that information regarding it could be given to 
stock owners. This information was released in the form of press 
notices and as a circular which gives instructions for the use of (his 
chemical in screwworm control. The treatment has been rather 
