270 
IOWA ACADEMY OF SCIENCES. 
In regard to the last plates poured it is a singular fact 
that but a very small development occurred, and this is 
strange since we had such an unusual development before 
running from 6,000 to 18,000 per cubic centimeter. 
In regard to the condition of the well it looks as though 
the water could easily have drained off from the surface, 
but nevertheless upon removing some of the boards from 
the top of the well I found that the water might easily 
have entered between the cracks of some of the boards. 
In fact I found moisture on the inside on the upper tile, 
showing the water had run down. One can readily see 
how B. coli-communis or other foreign organisms could get 
into the water. Gas was produced in one tube poured by 
Mr. Faurot and a slight amount in another. In this case 
we made the usual test. We also obtained gas from the 
first plates that I poured. 
The samples collected on October 29 were kept for forty 
days in the laboratory and then were examined by Mr. 
McKinley and Mr. Thomas with the following results: 
WELL. 
Depth. 
No. of germs. 
Briley Shallow Well 
45 feet. 
185 feet. 
200 
20 
10 
None. 
3 o 
340 
1. 000 
3 o 
Briley Deep Well 
Laboratory tap. Same source 
Kitchen Tap 
Skelton’s Well 
35 feet. 
185 feet. 
Peterson Deep Well 
Peterson’s Trough 
Pritchard Well.. 
170 feet. 
Various species were found. Some of these have been 
excluded as having no connection with Bacillus typhosus 
or B. coli-communis. On the other hand there are a number 
of species that belong to the typhosus group culturally so 
far as has been carried out. Our work was interrupted 
although cultures of all of the species were made and placed 
away for further study. Fire destroyed the entire labora- 
tory so no further study can be made. 
One peculiar pearly white Bacillus developed in consid- 
erable quantity, in fact at least three-fourths of the colon- 
