166 
IOWA ACADEMY OF SCIENCES. 
and with G. macropus from Cambridge, Mass., by Mr. B. M. 
Duggar. All were complete failures. The spring and summer 
were unusually dry. This probably accounts for the failures 
with Fluke crab and wild crab. Natural cultures of Boestelia 
on wild crab were rare. 
In the spring of 1895 one of us being on Long Island, N. Y., 
and the other at Ames, Iowa, we again undertook some experi- 
ments with G. macropus. We will speak first of the experi- 
ments on Long Island. They were conducted in the nursery of 
Isaac Hicks & Son at Westbury, N. Y. On May 18 th, four 
varieties were inoculated with New York G. macropus — Yellow 
Transparent, Red Astrachan, Ben Davis and Red Pippin, The 
first three were two-year-old nursery trees; the last was a large 
tree. Many leaves on one tree of each variety were smeared, 
both sides, with the gelatinous spore- masses of G. macropus. 
The results were as follows: Yellow Transparent showed no 
signs, whatever, of Boestelia. Both Red Astrachan and Ben 
Davis showed yellow spots which appeared like the beginning 
of Boestelia, but none of them developed. Red Pippin pro- 
duced the Boestelia, but the spores did not mature properly 
and the fungus presented a stunted appearance. On May 24th, 
six varieties were inoculated with Iowa G. macropus — Yellow 
Transparent, Red Astrachan, Ben Davis, Red Pippin, Maiden’s 
Blush and Wealthy. All were two-year- old nursery trees 
except the Red Pippin. One tree of each variety was inocu- 
lated as before. The results were as follows: Yellow Trans- 
parent and Red Pippin showed no signs of Boestelia. Red 
Astrachan and Ben Davis started Boestelia spots which never 
matured. Maiden’s Blush and Wealthy developed numerous 
Boestelia spots and matured the aecidiospores thoroughly. As 
no bags were used to cover the inoculated leaves, it can not be 
said positively that the Boestelia on Maiden’s Blush and 
Wealthy resulted from the Iowa G. macropus, but the condi- 
tions were such as to warrant the above conclusions. In the 
case of Red Pippin there can be no doubt as to which inocula- 
tion produced the Boestelia. A large tree which stood at con- 
siderable distance from the other inoculated trees, was inocu- 
lated on one side with New York G. macroptus and on the other 
side with Iowa G. macropus. The leaves of the branch inocu- 
lated with New York G. macropus, and a few other leaves in 
the immediate neighborhood, produced Boestelia while the 
remainder of the tree showed not a Boestelia spot. It is also 
