r.ie hybrid plajits of fis third, fo'JU'tla, and fifth generations con- 
tained either anabariiie or nicotine as vrell as a nixture of nicotine 
vrith anabasine , "but in such fixtures of the anabasine predoninatsd. 
Th.e conplex hybrids (nakhorka x 2-li.ucc. x toba.cco) inr?j:is.bly contained 
gjiabasine or a nixture of anabasine aiid nicotine, ""'o ne-" alkaloids 
rere found in any h^j/brids. As a r\ile the first- generation hybrid con- 
tains only one alkaloid, typical of one of the pc?rents; in the following 
generation splittin;; occurs aJid plants appear bearing mixt-'Jires of the 
alkaloids as vrell as plants containing only one or the other of the 
parental alkaloids. 
Spath and Ilesztler (143) in 1S37 reported that a minute quantity of 
levo-N' methylanabasine (picrate melting point 237-8°) v/as isolated fron 
1,800 grams of crude nicotine obtained from tobacco. 
Zul'ov (165) in 1959 reported on the inheritance of nicotine and 
anabasine in interspecific hybrids i^icotiana rurtica L. x iJ. glauca Orah. 
N. rustica contains nicotine only and i£. glauca . contains anabasine only. 
Comiaercia.. production of anabasine is based on the wild-grovring half- 
shrub Ar abas is aphylla ; in the youngest parts of this plant is contained 
up to 2.53 percent of anabasine. The demand for anabasine cannot be 
Trholly satisfied oT/ing to the low proportion of it in A. aphylla and to 
limited production. Breeding a new form rich in anabasine is, therefore, 
not only of theoretical, but also of practical interest. Of 105 h^.^brids 
studied, 41 contained only anabasine, 40 only nicotine, and 24 both 
alkaloids. a11 the anr.bar.ine-boajring plants morphologically belonged to 
typical mal±iorka ejad. r/ere self-fert:< le. The anab?.r:-ine content varied 
in these plants from 0.87 to 2.03 percent. The a:ialysis of leaves V7as 
conducted on unpruned plants. By me3ns of pruning and other agrotechinical 
treatment this percentage may be increased several times. As much as 
6.61 percent of anabasine was found in the leaves of one of the u:;pruned 
hybrids. 
Kostoff (62) in 1939 reported on the nicotine and citric a,cid con- 
tent in the progeny of the allo-polyploid hybrid i£. rustica L. x H. 
gla-uca GraJ-. Kostoff was of the opinion that this alio-poljTloid as 
well as the ba.ckcrosses axd other hybrids between K. rustica arid Ij_. 
glauca ma^;^ ansv/er the de^iands of industry for ai:abasine if breeding work 
with these plants be done on a somewhat laj'ger scale. Some of the allo- 
polyploid segregates, on the other hand, gror very rapidly and give a 
very large amoiont of green mass, provided suitable environmental con- 
ditions are secured for their growth. 
Allo-polyploid rTicot iana rust i ca- glauca is a plant interesting from 
an agricultural point of view, because it segregates forns v;ith larger 
amounts of anabasixie (1.986, 1.449, 1.395 percent, etc.) than the parental 
species N. gla-uca which, when grov.m under the same invironraental condi- 
tions, contained 0.837 percent. At the same time the offsr^ring contain 
a relatively large amo'jnt of citric acid. Further generations of some 
of the offsprinii should give the possibility to select forms with larger 
content of anabasine and citric acid. The populations produced from the 
backcrosses can be used for the same p-urpose. It should also be mentioned 
here that anabasine content can be increased about three times after de- 
capitation, as the analysis by Zukov showed. 
