c.l'c£-:.lcids ricotine, lupinine, collidir.e, a.id 1-atidine torrard -gold 
chloride, merc-oric chloride, Sraut ' s reagent, "ag:.^er's reagent, Mayer's 
reasent , picric acid, tari'om nitrate, chloroplatinic acid, silicotuiigstic 
acid, phosphoantiiiionic acid, phosphoTnolyodic acid, cadmium iodide, pctas- 
siun iodide + hydrogen peroxide, co'Calt thiocyar.ate , and Millon's re- 
agent. A taole is also ^iven sho'jins the oehavior of the different 
fractions to-.7ard most of these reagents and potassitin chronate in addi- 
tion. The most distinctive test is that with gold chloride; the crystals 
produced axe quite different from those forcied rrith nicotine. 
Sokolov (142) in 1937 claimed priority for some of these tests. 
CCCUEiGFCS CI JilJAIASIlS 
In addition to Ana'oasis aphylla , the follor^ing ^jlants have "oeen 
found to 2''ield anaoasine: 
Mcotiana tahacim (£path and Kes.itler, 147, in 1C37). 
H icotiana .glauca (Smith, 138, in 1935, and Zhiinura, 57, 53, in 1937 
ar.d 133e). 
Smith (138) reported that i^icotiana gl auca roots contain ahout 1 per- 
CEnt of anabasirie ; it also occ^^rs in thB dried leaver;. lot more than a 
trace of nicotine is present. J^jiatasine xras isolated oy digestion vrith. 
warm 1-percent hydrochloric acid, filtration, making alkaline, aiid ex- 
tracting rith ethyl ether. 
iComura (57) in 1937 write that -'ico tiana .^lauca may "become of com- 
mercial importance "because it conts-ins 0.74 percent of anabasine , 7.16 
percent of citric acid, and 7.90 percent of malic acid. The citric acid 
is concentrated mainly in the lorer leaves, vrhile anabasine is fo^^nd 
mainly in the yoxmg upper lea,ves. The amo'jjit of ara.ba.sine extracted with 
chloroform exceeds that extracted by petroleum ether by 100 percent. A 
considerable amount of anabasine is present in the stalks and roots. 
In 1933 Ihmvrs, (58) published additional information on the al".:aloid 
of Hicotiai'-a glauca, 
Anabasine was extracted from the plajit with 3-percent alcoholic 
ammonia solution. After distillation of the alcohol and solution of the 
residue in 5-percent lydrochZ.oric acid, the alkaloid was set free by 
alkali sation of the solution, anc extracted with et"ner. T"-^e ^'ield was 
24.4 'grails of pure alkaloid per 7.5 kilograms of plant (=0.32 percent). 
Stanley (150) in 1924 described Nicotian a glauca G-rah. as a glabrous 
shrub or sma.ll tree, 18 feet high or less. A native of Argentina and 
oruguay, it has become thorouglilj'- naturalized in come parts of North 
America. In the United States it is found from western Texas to southern 
California; in Mexico, from Sonora to Tamaulipas and Oaxaca. Its common 
names are as follows: 
